引用本文:杨婷, 陈星灼, 隋建枢, 王伟, 杨春苗, 彭泽, 耿广东, 张庆勤, 张素勤.六倍体小黑麦染色体的荧光原位杂交分析[J].西南大学学报(自然科学版),2019,41(4):42~48
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六倍体小黑麦染色体的荧光原位杂交分析
杨婷, 陈星灼, 隋建枢, 王伟, 杨春苗, 彭泽, 耿广东, 张庆勤, 张素勤1,2
1. 贵州大学 农学院, 贵阳 550025;2. 贵州省旱粮研究所, 贵阳 550006
摘要:
采用基因组原位杂交(GISH)和荧光原位杂交(FISH)技术对六倍体小黑麦进行了研究,以鉴定其含有的黑麦染色体的数目和结构特点,了解小黑麦基因组组成,为其在小麦遗传育种中的应用提供参考.分别以黑麦基因组DNA及pSc200和pSc250探针进行顺序GISH-FISH分析,结果显示该小黑麦含有21对染色体,来源于黑麦的2对染色体的长臂端部出现pSc200和pSc250信号,而另外5对染色体的两端部都有该信号.以Oligo-pSc119.2-1,pSc200和pSc250探针对小黑麦进行了双色FISH分析,发现小黑麦的Oligo-pSc119.2-1信号与黑麦基本相似,而pSc200和pSc250信号与黑麦的差异较大.以黑麦基因组DNA及Oligo-pAs1-1和Oligo-pSc119.2-1为探针对小黑麦进行顺序GISH-FISH分析,鉴定其基因组组成为AABBRR;同时发现小黑麦染色体的一些FISH信号发生了变化,其原因可能是小黑麦形成过程中染色体结构发生了改变,从而导致小黑麦的DNA重复序列呈现多态性.
关键词:  小黑麦  染色体  GISH  FISH  核型
DOI:10.13718/j.cnki.xdzk.2019.04.006
分类号:S503
基金项目:国家自然科学基金项目(31860380,31660390);国家“七大农作物育种”重点专项(2017YFD0100900);贵州省农业成果转化计划(黔科合成果[2016]4022号);贵州省普通高等学校重点实验室项目(黔教合KY字[2015]333);贵州省作物学省级重点学科建设计划(黔学位合字ZDXK[2014]8号);贵州大学自然科学专项(贵大专基合字[2013]01号).
Chromosomic Analysis of Hexaploid Triticale by Fluorescence in situ Hybridization
YANG Ting, CHEN Xing-zhuo, SUI Jian-shu, WANG Wei, YANG Chun-miao, PENG Ze, GENG Guang-dong, ZHANG Qing-qin, ZHANG Su-qin1,2
1. College of Agriculture, Guizhou University, Guiyang 550025, China;2. Guizhou Institute of Upland Crops, Guiyang 550006, China
Abstract:
In the present study, GISH (genomic in situ hybridization) and FISH (fluorescence in situ hybridization) techniques were employed to identify the chromosome number and structure of rye in hexaploid Triticale and to understand its genomic composition, which would provide reference for wheat genetics and breeding. Sequential GISH-FISH analysis with the rye genomic DNA, pSc200 and pSc250 probes showed that Triticale contained 21 pairs of chromosomes, of which 2 pairs derived from rye had pSc200 and pSc250 signals at the ends of the long arms, and other 5 pairs had the same signals at the ends of both arms. The two-color FISH analysis of Triticale with Oligo-pSc119.2-1, pSc200 and pSc250 probes showed that Oligo-pSc119.2-1 signals in Triticale was basically similar to those of the rye, but some signals of pSc200 and pSc250 probes were different between Triticale and the rye. Sequential GISH-FISH analysis of Triticale using the rye genomic DNA, Oligo-pAs1-1, and Oligo-pSc119.2-1 probes revealed that the genomic composition of Triticale was AABBRR. In addition, some FISH signals on Triticale chromosomes changed, which might be due to changes in the chromosome structure during Triticale formation. Therefore, DNA repeat sequences of Triticale were polymorphic.
Key words:  Triticale  chromosome  GISH  FISH  karyotype
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