引用本文:李学学, 黄钟杰, 威则日沙, 沈继秀, 张绍山, 刘圆.大理重楼和独龙重楼的野生资源分布和皂苷类成分评价[J].西南大学学报(自然科学版),2019,41(6):37~43
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大理重楼和独龙重楼的野生资源分布和皂苷类成分评价
李学学, 黄钟杰, 威则日沙, 沈继秀, 张绍山, 刘圆1,2,3
1. 西南民族大学 药学院, 成都 610041;2. 四川农业大学 农学院, 成都 611130;3. 西南民族大学 民族医药研究院, 成都 610041
摘要:
评价大理重楼和独龙重楼的野生资源分布情况和皂苷类成分.采用实地调研考察,并根据《中国药典》2015年版重楼项下3种重楼皂苷,增加重楼皂苷Ⅰ、纤细薯蓣皂苷、重楼皂苷H为指标,以课题组已经建立的ELSD-UPLC法测定大理重楼和独龙重楼中6种皂苷成分含量.结果表明:1)大理重楼除模式产地云南大理,在保山市腾冲市和贵州赫章县也发现了大理重楼的新分布,野生资源蕴藏量较少;独龙重楼只发现分布在云南省怒江州贡山县独龙江乡,野生资源蕴藏量更少;2)二者尚未被《中国药典》2015年版收载或者各省级药材标准中,但是其根茎在云南、贵州等各地民间也作为重楼药材使用;3)10批大理重楼根茎皂苷类成分总含量平均值高于药典规定标准1倍多,主要含有重楼皂苷Ⅶ和重楼皂苷H;4)在仅采集到的7批独龙重楼皂苷类成分总含量平均值达到药典规定标准,主要含有重楼皂苷Ⅰ和纤细偏诺皂苷,得出大理重楼和独龙重楼资源均稀少结论,建议高度重视开展人工繁育与资源保护工作;大理重楼根茎主要成分为偏诺皂苷("宫血宁"有效成分),独龙重楼根茎主要成分为薯蓣皂苷("抗病毒颗粒"消炎、抗菌、抗病毒有效成分),可为大理重楼和独龙重楼的省级药材标准建立和临床用药新药资源开发提供参考依据.
关键词:  大理重楼  独龙重楼  野生资源分布  皂苷类成分  评价
DOI:10.13718/j.cnki.xdzk.2019.06.006
分类号:R931.2
基金项目:国家重点研发计划项目(2018YFC1708005);阿坝州科技条件能力建设和技术示范推广项目(18TJPT0004);西南民族大学2019年研究生创新型科研项目硕士重点项目(CX2019SZ179).
On Distribution of the Wild Resources of Paris daliensis H. Li et V.G. Souku and P. dulongensis H. Li et S. Kuritap and Evaluation of Saponins in Them
LI Xue-xue, HUANG Zhong-jie, WEI Zerisha, SHEN Ji-xiu, ZHANG Shao-shan, LIU Yuan1,2,3
1. College of Pharmacy, Southwest Minzu University, Chengdu 610041, China;2. College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China;3. Ethnic Medicine Institute, Southwest Minzu University, Chengdu 610041, China
Abstract:
To evaluate the distribution of wild resources and steroid saponins of Paris daliensis H. Li et V.G. Souku and P. dulongensis H. Li et S. Kuritap, an on-the-spot investigation was made, and six kinds of steroid saponins, including the three polyphyllins listed in the 2015 edition of Chinese Pharmacopoeia, and polyphyllin I, gracillin, and polyphyllin H, in them were determined with the ELSD-UPLC method established by our research group. The results were as follows. 1) The model of P. daliensis H. Li et V.G. Souku originated from Dali, Yunnan province. In this investigation, this plant species was newly found to be distributed in Tengchong and Baoshan of Yunnan and in Hezhang of Guizhou as well. Its wild resources were not abundant. P. dulongensis was only found in Dulongjiang township, Nujiang prefecture, Yunnan province, with still less wild resource storage. 2) Neither P. daliensis or P. dulongensis was recorded in the 2015 edition of the Chinese Pharmacopoeia or in the standards of various provincial medicinal materials, but their rhizome was also used as Paridis Rhizoma among the local people in Yunnan and Guizhou. 3) The average total content of saponins in 10 batches of P. daliensis rhizoma was more than 1 time higher than the standard stipulated in Pharmacopoeia, mainly containing polyphyllin VⅡ and polyphyllin H. 4) The average value of the total content of saponins in only 7 batches of the P. dulongensis collected was up to the standard specified in Pharmacopoeia, and mainly containing polyphyllin I and slender steroidal pennogenin. It is concluded that P. daliensis and P. dulongensis are endangered species with rare resource storage, and their artificial multiplication and resource protection are necessary. The main component of P. daliensis is pennogenin, which is the active ingredient of "Gongxuening"; and that of P. dulongensis is dioscin, which is the active ingredient of the anti-inflammatory, antibacterial and antiviral medicine "Kangbingdu keli".
Key words:  Paris daliensis H. Li et V.G. Soukup  Paris dulongensis H. Li  wild resource distribution  saponins  evaluation
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