引用本文:唐运林, 吴燕燕, 顾偌铖, 邹祥明, 张祯, 牛小慧, 王泽乐, 陈洁, 吴玉娇, 李田, 李春峰, 韦俊宏, 潘国庆, 周泽扬.重庆地区草地贪夜蛾肠道细菌的分离鉴定[J].西南大学学报(自然科学版),2019,41(7):8~14
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重庆地区草地贪夜蛾肠道细菌的分离鉴定
唐运林, 吴燕燕, 顾偌铖, 邹祥明, 张祯, 牛小慧, 王泽乐, 陈洁, 吴玉娇, 李田, 李春峰, 韦俊宏, 潘国庆, 周泽扬1,2,3,4,5,6
1. 西南大学 家蚕基因组生物学国家重点实验室, 重庆 400715;2. 西南大学 农业部蚕桑生物学与遗传育种重点实验室, 重庆 400715;3. 西南大学 微孢子虫感染与防控重庆市重点实验室, 重庆 400715;4. 重庆市巫山县植保站, 重庆巫山 404700;5. 重庆市种子管理站, 重庆 401121;6.6. 重庆师范大学 生命科学学院, 重庆 401331
摘要:
为了解入侵重庆地区草地贪夜蛾(Spodoptera frugiperda)肠道所携带的细菌组成,以采集自巫溪、巫山地区玉米地里的草地贪夜蛾为材料,运用传统培养方法分离了其肠道优势细菌,并基于16S rDNA测序开展了优势菌的属水平鉴定,共获得了30个细菌分离株,经16S rDNA序列同源性分析,可归于5个属,分别为克雷伯氏菌属(Klebsiella)、不动杆菌属(Acinetobacter)、假单胞菌属(Pseudomonas)、肠杆菌属(Enterobacter)以及气单胞菌属(Aeromonas);其中,克雷伯氏菌属(Klebsiella)的丰度最高,占所有分离菌株的63%;假单胞属(Pseudomonas)和肠杆菌属(Enterobacter)菌株仅在巫山地区分离得到,气单胞菌属(Aeromonas)仅在巫溪地区分离得到.实验确定了入侵重庆地区草地贪夜蛾肠道优势细菌的种类及丰度,为后续研究草地贪夜蛾肠道微生物对宿主的生长发育以及迁飞等重要问题奠定了基础.
关键词:  重庆地区  草地贪夜蛾  肠道细菌  鉴定
DOI:10.13718/j.cnki.xdzk.2019.07.002
分类号:S433.4;Q969.436.5
基金项目:中央高校基本科研业务费团队项目(XDJK2018AA001);家蚕基因组生物学国家重点实验室自设课题(2019-03).
Isolation and Identification of Gut Bacteria of Spodoptera frugiperda That Migrated to Chongqing Area
唐运林, 吴燕燕, 顾偌铖, 邹祥明, 张祯, 牛小慧, 王泽乐, 陈洁, 吴玉娇, 李田, 李春峰, 韦俊宏, 潘国庆, 周泽扬1,2,3,4,5,6
1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;2. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Chongqing 400715, China;3. Chongqing Key Laboratory of Microsporidia Infection and Control, Chongqing 400715, China;4. Plant Protection Station of Wushan, Wushan Chongqing 404700, China;5. Crop Seed Management Station of Chongqing, Chongqing 401121, China;6.6. College of Life Sciences, Chongqing Normal University, Chongqing 401331, China
Abstract:
In order to understand the composition of bacteria in the guts of fall armyworm (FAW, Spodoptera frugiperda) that migrated to Chongqing, the dominant bacteria from the pest samples collected from maize fields in Wushan and Wuxi of Chongqing were isolated with the traditional culture method and identified with 16S rDNA sequencing. A total of 30 isolates of gut bacteria were obtained, and were clustered in 16S rDNA homology analyses into 5 taxa, i.e. Klebsiella, Acinetobacter, Pseudomonas, Enterobacter and Aeromonas. Of the 5 genera identified, Klebsiella had the highest abundance, accounting for 63% of the total. Pseudomonas and Enterobacter were isolated only in pest samples of Wushan, while Aeromonas was only found in those of Wuxi. In conclusion, this study has identified the generic composition of the intestinal bacteria of S. frugiperda that has migrated to Chongqing and determined their abundance, which may serve as a basis for future work on the impact of the intestinal microbes on the growth and development and migration of S. frugiperda.
Key words:  Chongqing area  fall armyworm (FAW, Spodoptera frugiperda)  gut bacteria  identification
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