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3种行道树叶片煤烟病真菌群落结构组成及多样性

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胡冲, 吴道军, 董国菊, 等. 3种行道树叶片煤烟病真菌群落结构组成及多样性[J]. 植物医学, 2022, (5): 23-33. doi: 10.13718/j.cnki.zwyx.2022.05.003
引用本文: 胡冲, 吴道军, 董国菊, 等. 3种行道树叶片煤烟病真菌群落结构组成及多样性[J]. 植物医学, 2022, (5): 23-33. doi: 10.13718/j.cnki.zwyx.2022.05.003
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HU Chong, WU Daojun, DONG Guoju, et al. Composition and Diversity of Fungal Community Structure of Sooty Mold in the Leaves of Three Street Trees[J]. PLANT HEALTH AND MEDICINE, 2022, (5): 23-33. doi: 10.13718/j.cnki.zwyx.2022.05.003
Citation: HU Chong, WU Daojun, DONG Guoju, et al. Composition and Diversity of Fungal Community Structure of Sooty Mold in the Leaves of Three Street Trees[J]. PLANT HEALTH AND MEDICINE, 2022, (5): 23-33. doi: 10.13718/j.cnki.zwyx.2022.05.003
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3种行道树叶片煤烟病真菌群落结构组成及多样性

  • 1. 重庆市风景园林科学研究院, 重庆 401329;

  • 2. 川渝共建乡土植物种质创新与利用重庆市重点实验室, 重庆 401329;

  • 3. 西南大学 植物保护学院, 重庆 400715

详细信息
    作者简介:

    胡冲,工程师,硕士研究生,主要从事病原菌鉴定及防控相关研究. .

  • 中图分类号: S432.4+4

Composition and Diversity of Fungal Community Structure of Sooty Mold in the Leaves of Three Street Trees

  • 1. Chongqing Landscape and Gardening Research Institute, Chongqing 401329, China;

  • 2. Chongqing Key Laboratory of Innovation and Utilization of Native Plant Germplasm in Sichuan and Chongqing, Chongqing 401329, China;

  • 3. College of Plant Protection, Southwest University, Chongqing 400715, China

  • 摘要: 本文利用高通量测序技术对重庆地区3种行道树香樟、天竺桂和黄葛树患煤烟病的叶片进行真菌扩增子测定,并对其真菌多样性进行生物信息学分析.结果发现,多样性指数显示在不同采样区的同一树种,其煤烟病真菌多样性和丰富度存在差异,如重庆市渝中区黄葛树和重庆市北碚区天竺桂煤烟病样本的Shannon指数和Simpson指数明显高于重庆市渝中区香樟和重庆市北碚区黄葛树煤烟病样本;检测样本中优势菌门是平均相对丰度为70.41%的子囊菌门;优势菌属分别是煤炱属、枝孢属、附球菌、短梗霉属,其占比分别是16.98%, 7.17%, 6.70%和6.23%;聚类分析和组间差异比较分析表明,同树种样本聚类距离更近,其差异菌群主要集中在相对丰度较低的非核心菌群中. FUNGuild解析显示,除天竺桂外,其他树种样本的煤烟病真菌群落均是腐生营养型真菌比例最高. 3种行道树煤烟病核心菌群种类并无明显差异,但不同栽植环境和树种种类可能是导致真菌结构、丰度和功能呈现显著差异的重要因素.
    Abstract: In this study, high-throughput sequencing technology was used to sequence the region of fungal amplicons from Cinnamomum camphora, Cinnamomum japonicum Sieb. and Ficus virens Aiton in Chongqing, and bioinformatic analysis was carried out for study of the fungal diversity. The results of diversity index showed that the diversity and richness of the sooty mold fungi in the same tree species in different sampling areas were different. For example, the shannon and simpson indexes of Ficus virens Aition in Daping Central Street of Yuzhong District in Chongqing and Cinnamomum japonicum sieb. in Luzuofu Street of Beibei District in Chongqing were significantly higher than those of Cinnamomum camphora in Heyuan Street of Yuzhong District in Chongqing and Ficus virens Aiton in Shuangyuan Street of Beibei District in Chongqing. The dominant phyla in the experimental samples is Ascomycota with an average relative abundance of 70.41%. The dominant genera were Capnodium, Cladosporium, Epicoccum and Aureobasidiumi, accounting for 16.98%, 7.17%, 6.70% and 6.23%, respectively. Cluster analysis and comparison between groups showed that the cluster distance of the same species was closer, and the difference was mainly concentrated in the non-core fungal community with low relative abundance. FUNGuild analysis showed that the proportion of saprophytic fungi was the highest in the fungal community of sooty mold disease in other tree species samples, except Cinnamomum japonicum Sieb. in Chongqing Academy of Agricultral Sciences of Jiulongpo District and Cinnamomum japonicum sieb. in Luzuofu Street of Beibei District in Chongqing. There was no significant difference in the species of core fungal communities among three street trees, but different planting environments and tree species may be important factors leading to significant differences in the structure, abundance and function of fungal communities.
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  • 收稿日期:  2022-09-15

3种行道树叶片煤烟病真菌群落结构组成及多样性

    作者简介: 胡冲,工程师,硕士研究生,主要从事病原菌鉴定及防控相关研究.
  • 1. 重庆市风景园林科学研究院, 重庆 401329;
  • 2. 川渝共建乡土植物种质创新与利用重庆市重点实验室, 重庆 401329;
  • 3. 西南大学 植物保护学院, 重庆 400715
  • 收稿日期:  2022-09-15
基金项目: 

摘要: 本文利用高通量测序技术对重庆地区3种行道树香樟、天竺桂和黄葛树患煤烟病的叶片进行真菌扩增子测定,并对其真菌多样性进行生物信息学分析.结果发现,多样性指数显示在不同采样区的同一树种,其煤烟病真菌多样性和丰富度存在差异,如重庆市渝中区黄葛树和重庆市北碚区天竺桂煤烟病样本的Shannon指数和Simpson指数明显高于重庆市渝中区香樟和重庆市北碚区黄葛树煤烟病样本;检测样本中优势菌门是平均相对丰度为70.41%的子囊菌门;优势菌属分别是煤炱属、枝孢属、附球菌、短梗霉属,其占比分别是16.98%, 7.17%, 6.70%和6.23%;聚类分析和组间差异比较分析表明,同树种样本聚类距离更近,其差异菌群主要集中在相对丰度较低的非核心菌群中. FUNGuild解析显示,除天竺桂外,其他树种样本的煤烟病真菌群落均是腐生营养型真菌比例最高. 3种行道树煤烟病核心菌群种类并无明显差异,但不同栽植环境和树种种类可能是导致真菌结构、丰度和功能呈现显著差异的重要因素.

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