草莓垫壳孢寄主范围测定及室内生物药剂筛选

马娅楠; 李继平; 郑果; 王立; 惠娜娜; 张自强; 黄振博

doi:10.13718/j.cnki.zwyx.2023.06.004

植物医学

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草莓垫壳孢寄主范围测定及室内生物药剂筛选

, , , , , ,

马娅楠, 李继平, 郑果, 等. 草莓垫壳孢寄主范围测定及室内生物药剂筛选[J]. 植物医学, 2023, (6): 28-35. doi: 10.13718/j.cnki.zwyx.2023.06.004

引用本文:

马娅楠, 李继平, 郑果, 等. 草莓垫壳孢寄主范围测定及室内生物药剂筛选[J]. 植物医学, 2023, (6): 28-35. doi: 10.13718/j.cnki.zwyx.2023.06.004

MA Yanan, LI Jiping, ZHENG Guo, et al. Determination of Host Range of Coniella fragariae and Indoor Bioagent Screening[J]. PLANT HEALTH AND MEDICINE, 2023, (6): 28-35. doi: 10.13718/j.cnki.zwyx.2023.06.004

Citation:

MA Yanan, LI Jiping, ZHENG Guo, et al. Determination of Host Range of Coniella fragariae and Indoor Bioagent Screening[J]. PLANT HEALTH AND MEDICINE, 2023, (6): 28-35. doi: 10.13718/j.cnki.zwyx.2023.06.004

草莓垫壳孢寄主范围测定及室内生物药剂筛选

1. 甘肃省农业科学院植物保护研究所, 兰州 730070;

2. 甘肃农业大学植物保护学院, 兰州 730070;

3. 国家药品监督管理局中药材及饮片质量控制重点实验室协同创新中心, 兰州 730070

详细信息

作者简介:
马娅楠,硕士研究生,主要从事植物病害及其综合防治研究. .

中图分类号: S432

Determination of Host Range of Coniella fragariae and Indoor Bioagent Screening

1. Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China;

2. College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China;

3. Collaborative Innovation Center of the Key Laboratory of Quality Control of Chinese Herbal Medicine and Decoction Pieces of the National Medical Products Administration, Lanzhou 730070, China

摘要: 为明确赤芍根腐病病原菌草莓垫壳孢（Coniella fragariae）的寄主范围并筛选有效的生物杀菌剂，本研究采用叶片刺伤接菌法，对赤芍、樱桃、牡丹等12种植物叶片进行致病性试验，并采用菌丝生长速率法测定了8种生物杀菌剂对C.fragariae的抑菌效果.结果表明，该病原菌可侵染赤芍、樱桃、牡丹、月季、苹果、石榴和核桃叶片，不侵染梨、山楂、竹、小叶黄杨、冬青叶片.0.3%丁子香酚对C.fragariae的抑制效果最好，EC₅₀仅为0.130 5 mg/L； 3%中生菌素次之，EC₅₀为4.225 6 mg/L； 8%宁南霉素、6%春雷霉素、4%嘧啶核苷类抗菌剂、10%多抗霉素和5.0亿多粘类芽孢杆菌抑菌效果较差，EC₅₀分别为63.586 8 mg/L，72.167 3 mg/L，293.900 3 mg/L，360.950 0 mg/L和405.708 1 mg/L； 24%井冈霉素对赤芍根腐病病原菌的抑制效果最差，抑菌效果不明显.该研究结果为田间赤芍根腐病的诊断及防治药剂的选择提供了理论依据.
- 赤芍 /
- 根腐病 /
- 草莓垫壳孢 /
- 寄主范围 /
- 生物杀菌剂
Abstract: In order to clarify the host range of Coniella fragariae, a pathogen of root rot disease of Paeonia veitchii Lynch, and screen effective biocides, the pathogenicity test was conducted on 12 species of plants, including P. veitchii, cherry and peony by leaf stabbing inoculation method, and the antifungal effect of 8 biological fungicides on C. fragariae was determined by mycelial growth rate. The results showed that the pathogen can infect the leaves of P. veitchii, cherry, peony, rose, apple, pomegranate and walnut, but could not infect the leaves of pear, hawthorn, bamboo, yellow poplar and holly. The inhibitory effect of 0.3% eugenol on C. fragariae was the best with only 0.130 5 mg/L of EC₅₀, and followed by 3% Zhongshengmycin with 4.225 6 mg/L of EC₅₀. The antifungal effect of 8% ningnanmycin, 6% kasugamycin, 4% pyrimidine nucleoside antimicrobials, 10% polycxins and 500 million Paenibacillus polymyxa was poor, with EC₅₀ value of 63.586 8 mg/L, 72.167 3 mg/L, 293.900 3 mg/L, 360.950 0 mg/L and 405.708 1 mg/L, respectively. The inhibitory effect of 24% Jinggangmycin on root rot of P. veitchii was the worst, and the inhibitory effect was not obvious. The results of this study provide theoretical basis for the diagnosis and control of P. veitchii root rot in the field.
- Paeonia veitchii Lynch /
- root rot disease /
- C. fragariae /
- host range /
- biological fungicides .

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草莓垫壳孢寄主范围测定及室内生物药剂筛选

作者简介: 马娅楠,硕士研究生,主要从事植物病害及其综合防治研究.
1. 甘肃省农业科学院植物保护研究所, 兰州 730070;

2. 甘肃农业大学植物保护学院, 兰州 730070;

3. 国家药品监督管理局中药材及饮片质量控制重点实验室协同创新中心, 兰州 730070

收稿日期: 2021-08-31

基金项目:

关键词:

摘要: 为明确赤芍根腐病病原菌草莓垫壳孢（Coniella fragariae）的寄主范围并筛选有效的生物杀菌剂，本研究采用叶片刺伤接菌法，对赤芍、樱桃、牡丹等12种植物叶片进行致病性试验，并采用菌丝生长速率法测定了8种生物杀菌剂对C.fragariae的抑菌效果.结果表明，该病原菌可侵染赤芍、樱桃、牡丹、月季、苹果、石榴和核桃叶片，不侵染梨、山楂、竹、小叶黄杨、冬青叶片.0.3%丁子香酚对C.fragariae的抑制效果最好，EC₅₀仅为0.130 5 mg/L； 3%中生菌素次之，EC₅₀为4.225 6 mg/L； 8%宁南霉素、6%春雷霉素、4%嘧啶核苷类抗菌剂、10%多抗霉素和5.0亿多粘类芽孢杆菌抑菌效果较差，EC₅₀分别为63.586 8 mg/L，72.167 3 mg/L，293.900 3 mg/L，360.950 0 mg/L和405.708 1 mg/L； 24%井冈霉素对赤芍根腐病病原菌的抑制效果最差，抑菌效果不明显.该研究结果为田间赤芍根腐病的诊断及防治药剂的选择提供了理论依据.

English Abstract

Determination of Host Range of Coniella fragariae and Indoor Bioagent Screening

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1. 甘肃省农业科学院植物保护研究所, 兰州 730070;
2. 甘肃农业大学植物保护学院, 兰州 730070;
3. 国家药品监督管理局中药材及饮片质量控制重点实验室协同创新中心, 兰州 730070

Received Date: 2021-08-31

Keywords:

Abstract: In order to clarify the host range of Coniella fragariae, a pathogen of root rot disease of Paeonia veitchii Lynch, and screen effective biocides, the pathogenicity test was conducted on 12 species of plants, including P. veitchii, cherry and peony by leaf stabbing inoculation method, and the antifungal effect of 8 biological fungicides on C. fragariae was determined by mycelial growth rate. The results showed that the pathogen can infect the leaves of P. veitchii, cherry, peony, rose, apple, pomegranate and walnut, but could not infect the leaves of pear, hawthorn, bamboo, yellow poplar and holly. The inhibitory effect of 0.3% eugenol on C. fragariae was the best with only 0.130 5 mg/L of EC₅₀, and followed by 3% Zhongshengmycin with 4.225 6 mg/L of EC₅₀. The antifungal effect of 8% ningnanmycin, 6% kasugamycin, 4% pyrimidine nucleoside antimicrobials, 10% polycxins and 500 million Paenibacillus polymyxa was poor, with EC₅₀ value of 63.586 8 mg/L, 72.167 3 mg/L, 293.900 3 mg/L, 360.950 0 mg/L and 405.708 1 mg/L, respectively. The inhibitory effect of 24% Jinggangmycin on root rot of P. veitchii was the worst, and the inhibitory effect was not obvious. The results of this study provide theoretical basis for the diagnosis and control of P. veitchii root rot in the field.