磷锑钼蓝共振瑞利散射法测定蛋白质
Resonance Rayleigh scattering method for the determination of protein with PSbMo heteropoly blue
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摘要: 在稀H2SO4介质中， 蛋白质与磷锑钼蓝结合而使共振瑞利散射(RRS)增强并产生一个新的RRS光谱. 以磷锑钼蓝(PSbMo heteropoly blue， PSbMo)-牛血清白蛋白(bovine serum albumin， BSA)为例， 考察了其相应的光谱特征、 影响因素和适宜的反应条件. 结果表明：当BSA的浓度在0～6.0 μg/mL范围内与RRS强度成正比. 方法对于测定蛋白质具有高的灵敏度， 对BSA、 人血清白蛋白(human serum albumin, HSA)、 α-糜蛋白酶的检测限(3σ)分别为0.021 μg/mL， 0.023 μg/mL和0.030 μg/mL. 实验了共存物质的干扰影响， 方法有较好的选择性， 用于合成样品的分析， 结果满意.Abstract: In the sulfuric acid media，some proteins react with P-Sb-Mo heteropoly blue to form the combined products，lead to the enhancement of RRS and a new RRS spectrum was produced. The intensity of RRS is directly proportional to the concentration of protein in the range 0～6.0 μg/mL for BSA ，0～4.0 μg/mL for HSA and 0.5～5.0 μg/mL for α-chyhotrypsi，the detection limits(3σ) are 0.021 μg/mL for BSA，0.023 μg/mL for HSA and 0.030 μg/mL for α-chynotrypsin respectively. The method has good selectivity and applied to determination of BSA in synthetic samples with satisfaction results
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Key words:
- protein /
- PSbMo heteropoly blue /
- Resonance Rayleigh scattering .
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