核酸某些碱性三苯甲烷染料体系的 RRS及其分析应用
Resonance Rayleigh Scattering of the Determination of Nucleic Acid-Basic Triphenylmethane Dyes System and Its Analytical Application
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摘要: 在Tris缓冲溶液中，核酸能与某些碱性三苯甲烷染料如乙基紫(Ethyl Violet, EV)、结晶紫(Crystal Violet, CV)和甲基紫(Methyl V iolet, MV)结合而使共振瑞利散射(RRS)急剧增强并产生新的RRS光谱.以鲱鱼精DNA (Herring Sperm DNA，hsDNA)为例考察了其相应的光谱特征、影响因素和适宜的反应条件，体系均在hsDNA浓度为0～2.0 μg/mL时与散射强度呈直线关系，方法具有较高的灵敏度，其检出限(3σ)分别为4.7 ng/mL(EV-hsDNA体系)， 13.0 ng/mL(CV-hsDNA体系)和12.2 ng/mL(MV-hsDNA 体系).考察了某些共存物质的影响，并用于合成样品中核酸的测定，获得了满意的结果.Abstract: In a medium of Tris buffer solution, nucleic acids can bind with some basic triphenylmethane dyes including ethyl violet(EV), crystal v iolet(CV) and methyl violet(MV) to form new products, resulting in the great enh ancement of RRS and new RRS spectra. The intensity of RRS is directly proportion al to the concentration of hsDNA in the range of 0～2.0 ug/mL for all system. T he method has fairly high sensitivity and the detecting limits of hsDNA (3σ) ar e 4.7ng/mL for EV-hsDNA system, 13 ng/mL for CV-hsDNA system and 12.2 ng/mL for MV-hsDNA system,respectively. The influence of some coexisiting substance w as investigated and the method was applied to the determination of trace amounts of nucleic acid in synthetic samples with satisfactory results.
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Key words:
- nucleic acid /
- basic triphenylmethane dyes /
- Resonance R ayleigh scattering .
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