Abstract: ISSR was used to analyze the genetic relationship among mulberry varieties from Guangxi Prov‐ince .The study had shown that there were four groups of mulberry species had closer genetic relationship . The first group includes Liu xu NO.7 ,Ling tai NO.3 ,Gong tong NO.5 ,Da si NO.12 ,Chi tang NO.1 , Liu xu NO.2 ,Na xue NO.8 ,Na chen NO.2 ,Na xue NO.14 ,Xiao dong NO.1 ,Ban chao NO.1 ,Feng wu NO.1 ,Yong xin NO.3 ,Yong xin NO.11 ,Gong tong NO.9 ,Tai ping NO.2 ,Sha you NO.4 and Ban tun che NO.2 .There were 5 kinds of mulberry varieties in the second group ,they were Da si te hao ,Feng wu NO.5 ,Chi tang NO.4 ,Tai ping xin NO.1 ,Gong cheng NO.4 .The third group was composed of Da si NO.5 ,Ling tai NO.1 ,Gong jiang NO.1 ,Wei sheng NO.4 ,Ling tai NO.2 ,Ban luo NO.1 ,Na lou NO.14 ,Gong tong NO.4 .The fourth group includes Qin zhou sang ,Da Xin bai sang ,Guang Xi ji sang , Quan zhou chang sui sang ,Huan jiang NO.1 ,Qin zhou chang guo sang ,Gui NO.772 ,Wei zhou dao bai sang ,Yong xin jing sang NO.12 ,Long lin gui sang ,Long lin meng sang .The other result was that Morus alba is large genetic variation .Morus atropurpurea had close relationship with the Morus alba ,which sup‐ported a fact that Morus atropurpurea was a variety of Morus alba in Flora China and GenBank .The ge‐netic background between wild mulberry species and cultivated mulberry was different .The genetic back‐ground among Qinzhou sang ,Gui NO.772 ,Yongxin jingsang NO.12 tended to wild mulberry ,they had some advantages ,for example ,resistance ,the characteristics should be used in hybrid breeding .
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