几种忍冬属植物花粉离体萌发研究
摘要: 为了探讨忍冬属(Lonicera Linn.)植物花粉萌发特性,为有性杂交奠定基础,研究了不同质量分数的蔗糖溶液(15%,20%,25%,30%)对贯月忍冬(L. sempervirens Linn.)离体花粉的萌发作用,分别进行了不同花粉采集时间(8:00~18:00)和不同培养温度(15℃,20℃,25℃,30℃)对4种离体花粉的萌发影响.结果表明30%蔗糖+0.01%硼酸的培养液中贯月忍冬花粉萌发迅速、萌发率高,可以作为测定花粉萌发的培养基;贯月忍冬、“格雷姆”忍冬(L. periclymenum “Geleimu”)和滇西忍冬(L. buchananii Lace)早晨8:00采集的花粉萌发率高,红白忍冬(L. j aponica var Chinensis)18:00采集的花粉萌发率显著高于其他时间;25℃条件下贯月忍冬离体花粉萌发速度快,4 h萌发率为95.1%;30℃条件下“京红久”忍冬(L.× heckrottii)、滇西忍冬和红白忍冬萌发率高,4 h萌发率分别为12.4%,84.7%,34.1%;贯月忍冬、“京红久”忍冬、滇西忍冬离体花粉在25℃和30℃条件下差异不具有统计学意义,红白忍冬差异具有统计学意义.不同品种间花粉萌发率存在明显差异,贯月忍冬的花粉萌发率最高.
Study on Pollen Germination in vit ro for Several Species of Lonicera
Abstract: In order to investigate pollen germination characteristics of Lonicera Linn. species and lay a foundation for their sexual hybridization ,the authors studied in vitro pollen germination of L. sempervi‐rens at different sucrose concentrations (15% ,20% ,25% and 30% ) .Four kinds of pollen were collected at different times of the day (8 :00-18 :00) and let to germinate in v itro at different temperatures (15 ℃ , 20 ℃ ,25 ℃ and 30 ℃) .The results showed that the pollen of L. sempervirens germinated quickly in the culture liquid containing 30% sucrose+ 0.01% boric acid with a high germination rate ,which could be used as a pollen germination medium .The pollen of L. sempervirens Linn. ,L. periclymenum“Geleimu”and L. buchananii Lace. had high germination rate w hen collected at 8:00 a. m. ,and the pollen of L. j a‐ponica var. chinensis collected at 18 :00 p. m. had significantly higher germination rate than that collected at other times .Under the condition of 25 ℃ ,the pollen of L. sempervirens Linn. germinated in vitro quickly ,with a germination rate of 95.1% in 4 h .The pollen of L. × heckrottii ,L. buchananii Lace and L. j aponica var. chinensis gave a high germination rate at 30 ℃ ,being 12.4% ,84.7% and 12.4% ,re‐spectively ,in 4 h .No significant difference was noticed between 25 ℃ and 30 ℃ in pollen germination rate of L. sempervirens ,L. × heckrottii and L. buchananii Lace ,while a significant difference was recorded for L. j aponica var. chinensis .Pollen germination rate was significantly different between different varie‐ties ,being the highest for L. sempervirens Linn .