家蚕抗核型多角体病分子标记筛选的遗传基础
GENETIC BASIS OF CONSTRUCTING F2 POPULATIONS AND NEAR ISOGENIC LINES OF BmNPV RESISTANCE MOLECULAR MARKERS
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摘要: 家蚕对NPV抵抗性主要受一对位于常染色体上显性基因控制，又为不完全显性，Z染色体上有微效修饰基因，有偏父遗传现象存在。根据其遗传规律的特殊性，提出常染色体上NN主基因和性染色体上Z+Z+修饰基因控制假设，并得到试验验证。利用近等基因系寻找抗性分子标记，其交配方式不同，分别可构建NN，Z+Z+，NNZ+Z+3种近等基因系。同时提出F2群体寻找分子标记的交配方式。
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关键词:
- 家蚕,抗NPV,分子标记,交配设计
Abstract: NPV resistance in silkworm (Bombyx mori) is mainly regulated by a pair of dominant genes on an autosome. It is, however, not complete dominance, becau se minor modifer genes are present on Chromosome Z and a patroclinal tendency is shown in inheritance. Based on the hereditary properties of NPV resistance, a h ypothesis was proposed and confirmed that the resistance is controlled by NN mai n gene on an autosome and Z+ Z+ minor genes on the sex chromosome. By diffe rent mating methods, three near isogenic lines, i.e. NN, Z+ Z+, and NN Z+ Z+, which are used in search for molecular markers of resistance, were constructed . A mating model is put forward for checking molecular markers using the F2 po pulations. -
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