牛血清白蛋白共振瑞利散射和共振非线性散射法测定硫酸软骨素

刘健,刘忠芳,胡小莉,刘绍璞

西南大学学报（自然科学版）

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牛血清白蛋白共振瑞利散射和共振非线性散射法测定硫酸软骨素

刘健,刘忠芳,胡小莉,刘绍璞. 牛血清白蛋白共振瑞利散射和共振非线性散射法测定硫酸软骨素[J]. 西南大学学报（自然科学版）, 2010, 32(3).

引用本文:

刘健,刘忠芳,胡小莉,刘绍璞. 牛血清白蛋白共振瑞利散射和共振非线性散射法测定硫酸软骨素[J]. 西南大学学报（自然科学版）, 2010, 32(3).

Determination of Chondroitin Sulfate by Resonance Rayleigh Scattering and Resonance Non-linear Scattering Method with Bovine Serum Albumin[J]. Journal of Southwest University Natural Science Edition, 2010, 32(3).

Citation:

Determination of Chondroitin Sulfate by Resonance Rayleigh Scattering and Resonance Non-linear Scattering Method with Bovine Serum Albumin[J]. Journal of Southwest University Natural Science Edition, 2010, 32(3).

牛血清白蛋白共振瑞利散射和共振非线性散射法测定硫酸软骨素

刘健,刘忠芳,胡小莉,刘绍璞

西南大学环境化学研究所,重庆,400715

Determination of Chondroitin Sulfate by Resonance Rayleigh Scattering and Resonance Non-linear Scattering Method with Bovine Serum Albumin

摘要: 在pH=2.8～4.0的BR缓冲溶液中,硫酸软骨素(CS)由于磺酸基离解而成为带多个负电荷的大阴离子,而低于其等电点(pI=4.7)的牛血清白蛋白(BSA)则以带正电荷的大阳离子存在,两者之间可结合形成复合物.此时将引起共振瑞利散射(RRS)显著增强,并产生新的RRS光谱,其最大散射波长位于304 nm处.与此同时,它的二级散射(SOS)和倍频散射(FDS)也明显增强,且最大SOS和FDS分别位于471 nm和292 nm.散射增强的强度(△I_(RRS)、△I_(SOS)和△I_(FDS)与CS浓度在一定范围内成正比,可用于CS的定量测定.对于CS的检出限(3σ)分别为2.0 ng/mL(RRS),2.9 ng/mL(SOS)和13.2 ng/mL(FDS).研究了适宜的反应条件,考察了共存物质的影响,表明方法有较好的选择性,可用于市售滴眼液中硫酸软骨素含量的测定.
- 共振瑞利散射,共振非线性散射,硫酸软骨素,牛血清白蛋白
Abstract: In a Britton-Robinson (BR) buffer solution of pH 2.8--4.0, chondroitin sulfate (CS), which carries negative charge by the ionization of sulfonie group, reacted with bovine serum albumin, which car-ries positive charge through electrostatic force and hydrophobic interaction, to form a complex, resulting in a significant enhancement of resonance Rayleigh scattering (RRS) and the appearance of a new RRS spectrum with the maximum wavelength at 304 nm.At the same time, the second-order scattering (SOS) and frequency-doubling scattering (FDS) were enhanced obviously with a peak at 471 nm and 392 nm, re-spectively.The intensity of scattering (△I_(RRS), △I_(SOS) and △I_(FDS)) was directly proportional to the concentra-tion of CS in a certain range.When used in CS determination, this method exhibited high sensitivity, with a detection limit of 2.0(RRS), 2.9(SOS) and 13.2(FDS) ng/mL.The optimum conditions for the reac-tion and the influencing factors were investigated.The interference of foreign substances was examined and this method showed a good selectivity.The method can be applied satisfactorily to the determination of CS in eye drops.

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牛血清白蛋白共振瑞利散射和共振非线性散射法测定硫酸软骨素

刘健,刘忠芳,胡小莉,刘绍璞

西南大学环境化学研究所,重庆,400715

关键词:

共振瑞利散射,共振非线性散射,硫酸软骨素,牛血清白蛋白

摘要: 在pH=2.8～4.0的BR缓冲溶液中,硫酸软骨素(CS)由于磺酸基离解而成为带多个负电荷的大阴离子,而低于其等电点(pI=4.7)的牛血清白蛋白(BSA)则以带正电荷的大阳离子存在,两者之间可结合形成复合物.此时将引起共振瑞利散射(RRS)显著增强,并产生新的RRS光谱,其最大散射波长位于304 nm处.与此同时,它的二级散射(SOS)和倍频散射(FDS)也明显增强,且最大SOS和FDS分别位于471 nm和292 nm.散射增强的强度(△I_(RRS)、△I_(SOS)和△I_(FDS)与CS浓度在一定范围内成正比,可用于CS的定量测定.对于CS的检出限(3σ)分别为2.0 ng/mL(RRS),2.9 ng/mL(SOS)和13.2 ng/mL(FDS).研究了适宜的反应条件,考察了共存物质的影响,表明方法有较好的选择性,可用于市售滴眼液中硫酸软骨素含量的测定.

English Abstract

Determination of Chondroitin Sulfate by Resonance Rayleigh Scattering and Resonance Non-linear Scattering Method with Bovine Serum Albumin

西南大学环境化学研究所,重庆,400715

Keywords:

共振瑞利散射,共振非线性散射,硫酸软骨素,牛血清白蛋白

Abstract: In a Britton-Robinson (BR) buffer solution of pH 2.8--4.0, chondroitin sulfate (CS), which carries negative charge by the ionization of sulfonie group, reacted with bovine serum albumin, which car-ries positive charge through electrostatic force and hydrophobic interaction, to form a complex, resulting in a significant enhancement of resonance Rayleigh scattering (RRS) and the appearance of a new RRS spectrum with the maximum wavelength at 304 nm.At the same time, the second-order scattering (SOS) and frequency-doubling scattering (FDS) were enhanced obviously with a peak at 471 nm and 392 nm, re-spectively.The intensity of scattering (△I_(RRS), △I_(SOS) and △I_(FDS)) was directly proportional to the concentra-tion of CS in a certain range.When used in CS determination, this method exhibited high sensitivity, with a detection limit of 2.0(RRS), 2.9(SOS) and 13.2(FDS) ng/mL.The optimum conditions for the reac-tion and the influencing factors were investigated.The interference of foreign substances was examined and this method showed a good selectivity.The method can be applied satisfactorily to the determination of CS in eye drops.