Natural History Museum Bern[EB/OL]. World Spider Catalog Version 25.0. [2024-06-24]. http://wsc.nmbe.ch.
VOLLRATH F. Strength and Structure of Spiders' Silks[J]. Journal of Biotechnology, 2000, 74(2): 67-83.
HTUT K Z, ALICEA-SERRANO A M, SINGLA S, et al. Correlation between Protein Secondary Structure and Mechanical Performance for the Ultra-Tough Dragline Silk of Darwin's Bark Spider[J]. Journal of the Royal Society Interface, 2021, 18(179): 20210320. doi: 10.1098/rsif.2021.0320
PRINCE J T, MCGRATH K P, DIGIROLAMO C M, et al. Construction, Cloning, and Expression of Synthetic Genes Encoding Spider Dragline Silk[J]. Biochemistry, 1995, 34(34): 10879-10885. doi: 10.1021/bi00034a022
FAHNESTOCK S R, BEDZYK L A. Production of Synthetic Spider Dragline Silk Protein in Pichia Pastoris[J]. Applied Microbiology and Biotechnology, 1997, 47(1): 33-39. doi: 10.1007/s002530050884
JANSSON R, LAU C H, ISHIDA T, et al. Functionalized Silk Assembled from a Recombinant Spider Silk Fusion Protein (Z-4RepCT) Produced in the Methylotrophic Yeast Pichia Pastoris[J]. Biotechnology Journal, 2016, 11(5): 687-699. doi: 10.1002/biot.201500412
SCHELLER J, GVHRS K H, GROSSE F, et al. Production of Spider Silk Proteins in Tobacco and Potato[J]. Nature Biotechnology, 2001, 19(6): 573-577. doi: 10.1038/89335
PENG C A, RUSSO J, GRAVGAARD C, et al. Spider Silk-Like Proteins Derived from Transgenic Nicotiana Tabacum[J]. Transgenic Research, 2016, 25(4): 517-526. doi: 10.1007/s11248-016-9949-1
LAZARIS A, ARCIDIACONO S, HUANG Y, et al. Spider Silk Fibers Spun from Soluble Recombinant Silk Produced in Mammalian Cells[J]. Science, 2002, 295(5554): 472-476. doi: 10.1126/science.1065780
WILLIAMS D. Sows' Ears, Silk Purses and Goats' Milk: New Production Methods and Medical Applications for Silk[J]. Medical Device Technology, 2003, 14(5): 9-11.
HEIDEBRECHT A, SCHEIBEL T. Recombinant Production of Spider Silk Proteins[J]. Advances in Applied Microbiology, 2013, 82: 115-153.
MIAO Y G, ZHANG Y S, NAKAGAKI K, et al. Expression of Spider Flagelliform Silk Protein in Bombyx Mori Cell Line by a Novel Bac-to-Bac/BmNPV Baculovirus Expression System[J]. Applied Microbiology and Biotechnology, 2006, 71(2): 192-199. doi: 10.1007/s00253-005-0127-2
ALTMANN F, STAUDACHER E, WILSON I B H, et al. Insect Cells as Hosts for the Expression of Recombinant Glycoproteins[J]. Glycoconjugate Journal, 1999, 16(2): 109-123. doi: 10.1023/A:1026488408951
MI J P, ZHOU Y Z, MA S Y, et al. High-Strength and Ultra-Tough Whole Spider Silk Fibers Spun from Transgenic Silkworms[J]. Matter, 2023, 6(10): 3661-3683. doi: 10.1016/j.matt.2023.08.013
ZHANG Y S, HU J H, MIAO Y G, et al. Expression of EGFP-Spider Dragline Silk Fusion Protein in BmN Cells and Larvae of Silkworm Showed the Solubility is Primary Limit for Dragline Proteins Yield[J]. Molecular Biology Reports, 2008, 35(3): 329-335. doi: 10.1007/s11033-007-9090-6
DE C BITTENCOURT D M, OLIVEIRA P, MICHALCZECHEN-LACERDA V A, et al. Bioengineering of Spider Silks for the Production of Biomedical Materials[J]. Frontiers in Bioengineering and Biotechnology, 2022(10): 958486.
MI J P, LI X, NIU S W, et al. High-Strength and Ultra-Tough Supramolecular Polyamide Spider Silk Fibers Assembled via Specific Covalent and Reversible Hydrogen Bonds[J]. Acta Biomaterialia, 2024, 176: 190-200. doi: 10.1016/j.actbio.2024.01.004
SCHACHT K, SCHEIBEL T. Processing of Recombinant Spider Silk Proteins into Tailor-Made Materials for Biomaterials Applications[J]. Current Opinion in Biotechnology, 2014, 29: 62-69. doi: 10.1016/j.copbio.2014.02.015
BABB P L, LAHENS N F, CORREA-GARHWAL S M, et al. The Nephila Clavipes Genome Highlights the Diversity of Spider Silk Genes and Their Complex Expression[J]. Nature Genetics, 2017, 49(6): 895-903. doi: 10.1038/ng.3852
RISING A, JOHANSSON J. Toward Spinning Artificial Spider Silk[J]. Nature Chemical Biology, 2015, 11(5): 309-315. doi: 10.1038/nchembio.1789
HAYASHI C Y, SHIPLEY N H, LEWIS R V. Hypotheses that Correlate the Sequence, Structure, and Mechanical Properties of Spider Silk Proteins[J]. International Journal of Biological Macromolecules, 1999, 24(2-3): 271-275. doi: 10.1016/S0141-8130(98)00089-0
XU M, LEWIS R V. Structure of a Protein Superfiber: Spider Dragline Silk[J]. Proceedings of the National Academy of Sciences of the United States of America, 1990, 87(18): 7120-7124.
HINMAN M B, LEWIS R V. Isolation of a Clone Encoding a Second Dragline Silk Fibroin. Nephila Clavipes Dragline Silk is a Two-Protein Fiber[J]. Journal of Biological Chemistry, 1992, 267(27): 19320-19324. doi: 10.1016/S0021-9258(18)41777-2
BROOKS A E, STEINKRAUS H B, NELSON S R, et al. An Investigation of the Divergence of Major Ampullate Silk Fibers from Nephila Clavipes and Argiope Aurantia[J]. Biomacromolecules, 2005, 6(6): 3095-3099. doi: 10.1021/bm050421e
DOBLHOFER E, HEIDEBRECHT A, SCHEIBEL T. To Spin or Not to Spin: Spider Silk Fibers and More[J]. Applied Microbiology and Biotechnology, 2015, 99(22): 9361-9380. doi: 10.1007/s00253-015-6948-8
LI J T, LI S T, HUANG J Y, et al. Spider Silk-Inspired Artificial Fibers[J]. Advanced Science, 2022, 9(5): 2103965. doi: 10.1002/advs.202103965
HAYASHI C Y, BLACKLEDGE T A, LEWIS R V. Molecular and Mechanical Characterization of Aciniform Silk: Uniformity of Iterated Sequence Modules in a Novel Member of the Spider Silk Fibroin Gene Family[J]. Molecular Biology and Evolution, 2004, 21(10): 1950-1959. doi: 10.1093/molbev/msh204
SPONNER A, VATER W, ROMMERSKIRCH W, et al. The Conserved C-Termini Contribute to the Properties of Spider Silk Fibroins[J]. Biochemical and Biophysical Research Communications, 2005, 338(2): 897-902. doi: 10.1016/j.bbrc.2005.10.048
GUERETTE P A, GINZINGER D G, WEBER B H F, et al. Silk Properties Determined by Gland-Specific Expression of a Spider Fibroin Gene Family[J]. Science, 1996, 272(5258): 112-115. doi: 10.1126/science.272.5258.112
SAVAGE K N, GOSLINE J M. The Effect of Proline on the Network Structure of Major Ampullate Silks as Inferred from Their Mechanical and Optical Properties[J]. The Journal of Experimental Biology, 2008, 211(12): 1937-1947. doi: 10.1242/jeb.014217
EISOLDT L, SMITH A, SCHEIBEL T. Decoding the Secrets of Spider Silk[J]. Materials Today, 2011, 14(3): 80-86. doi: 10.1016/S1369-7021(11)70057-8
KONO N, NAKAMURA H, OHTOSHI R, et al. Orb-Weaving Spider Araneus Ventricosus Genome Elucidates the Spidroin Gene Catalogue[J]. Scientific Reports, 2019(9): 8380.
KONO N, NAKAMURA H, MORI M, et al. MulticomponentNature Underlies the Extraordinary Mechanical Properties of Spider Dragline Silk[J]. Proceedings of the National Academy of Sciences of the United States of America, 2021, 118(31): 1-10.
GARB J E, HANEY R A, SCHWAGER E E, et al. The Transcriptome of Darwin's Bark Spider Silk Glands Predicts Proteins Contributing to Dragline Silk Toughness[J]. Communications Biology, 2019(2): 275.
MALAY A D, CRAIG H C, CHEN J M, et al. Complexity of Spider Dragline Silk[J]. Biomacromolecules, 2022, 23(5): 1827-1840. doi: 10.1021/acs.biomac.1c01682
BABB P L, GREGORIC M, LAHENS N F, et al. Characterization of the Genome and Silk-Gland Transcriptomes of Darwin's Bark Spider (Caerostris darwini)[J]. PLoS One, 2022, 17(6): e0268660. doi: 10.1371/journal.pone.0268660
TIAN L Y, MENG Q, LIN Y. Expression and Characterization of Chimeric Spidroins from Flagelliform-Aciniform Repetitive Modules[J]. Biopolymers, 2020, 111(12): e23404. doi: 10.1002/bip.23404
LI J X, YANG G Z, LI X, et al. Nanoassembly of Spider Silk Protein Mediated by Intrinsically Disordered Regions[J]. International Journal of Biological Macromolecules, 2024, 271: 132438. doi: 10.1016/j.ijbiomac.2024.132438
STELLWAGEN S D, BURNS M. Repeat Variation Resolves a Complete Aggregate Silk Sequence of Bolas Spider Mastophora Phrynosoma[J]. Integrative and Comparative Biology, 2021, 61(4): 1450-1458. doi: 10.1093/icb/icab048
COLLIN M A, CLARKE T H, AYOUB N A, et al. Evidence from Multiple Species that Spider Silk Glue Component ASG2 is a Spidroin[J]. Scientific Reports, 2016(6): 21589.
WEN R, WANG K K, YANG D, et al. The Novel Aciniform Silk Protein (AcSp2-V2) Reveals the Unique Repetitive Domain with High Acid and Thermal Stability and Self-Assembly Capability[J]. International Journal of Biological Macromolecules, 2022, 202: 91-101. doi: 10.1016/j.ijbiomac.2021.12.088
HU X Y, LAWRENCE B, KOHLER K, et al. Araneoid Egg Case Silk: A Fibroin with Novel Ensemble Repeat Units from the Black Widow Spider, Latrodectus Hesperus[J]. Biochemistry, 2005, 44(30): 10020-10027. doi: 10.1021/bi050494i
SHANAFELT M, LARRACAS C, DYRNESS S, et al. Egg Case Protein 3: A Constituent of Black Widow Spider Tubuliform Silk[J]. Molecules, 2021, 26(16): 5088. doi: 10.3390/molecules26165088
WEN R, WANG K K, MENG Q. Two Novel Tubuliform Silk Gene Sequences from Araneus ventricosus Provide Evidence for Multiple Loci in Genome[J]. International Journal of Biological Macromolecules, 2020, 160: 806-813. doi: 10.1016/j.ijbiomac.2020.05.141
BLASINGAME E, TUTON-BLASINGAME T, LARKIN L, et al. Pyriform Spidroin 1, a Novel Member of the Silk Gene Family that Anchors Dragline Silk Fibers in Attachment Discs of the Black Widow Spider, Latrodectus hesperus[J]. Journal of Biological Chemistry, 2009, 284(42): 29097-29108. doi: 10.1074/jbc.M109.021378
GEURTS P, ZHAO L, HSIA Y, et al. Synthetic Spider Silk Fibers Spun from Pyriform Spidroin 2, a Glue Silk Protein Discovered in Orb-Weaving Spider Attachment Discs[J]. Biomacromolecules, 2010, 11(12): 3495-3503. doi: 10.1021/bm101002w
HAYASHI C Y, LEWIS R V. Evidence from Flagelliform Silk cDNA for the Structural Basis of Elasticity and Modular Nature of Spider Silks[J]. Journal of Molecular Biology, 1998, 275(5): 773-784. doi: 10.1006/jmbi.1997.1478
DOS SANTOS-PINTO J R A, ARCURI H A, ESTEVES F G, et al. Spider Silk Proteome Provides Insight into the Structural Characterization of Nephila Clavipes Flagelliform Spidroin[J]. Scientific Reports, 2018(8): 14674.
LI X, QI X M, CAI Y M, et al. Customized Flagelliform Spidroins Form Spider Silk-Like Fibers at pH 8.0 with Outstanding Tensile Strength[J]. ACS Biomaterials Science & Engineering, 2022, 8(1): 119-127.
JORGE I, RUIZ V, LAVADO-GARCÍA J, et al. Expression of Spidroin Proteins in the Silk Glands of Golden Orb-Weaver Spiders[J]. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, 2022, 338(4): 241-253. doi: 10.1002/jez.b.23117
PAWAR K, WELZEL G, HAYNL C, et al. Recombinant Spider Silk and Collagen-Based Nerve Guidance Conduits Support Neuronal Cell Differentiation and Functionality in Vitro[J]. ACS Applied Bio Materials, 2019, 2(11): 4872-4880. doi: 10.1021/acsabm.9b00628
CHEN G F, LIU X Q, ZHANG Y L, et al. Full-Length Minor Ampullate Spidroin Gene Sequence[J]. PLoS One, 2012, 7(12): e52293. doi: 10.1371/journal.pone.0052293
COLGIN M A, LEWIS R V. SpiderMinor Ampullate Silk Proteins Contain New Repetitive Sequences and Highly Conserved Non-Silk-Like "Spacer Regions"[J]. Protein Science, 1998, 7(3): 667-672. doi: 10.1002/pro.5560070315
WIDMAIER D M, TULLMAN-ERCEK D, MIRSKY E A, et al. Engineering the Salmonella Type Ⅲ Secretion System to Export Spider Silk Monomers[J]. Molecular Systems Biology, 2009, 5(1): 309. doi: 10.1038/msb.2009.62
TIAN M Z, LEWIS R V. Molecular Characterization and Evolutionary Study of Spider Tubuliform (Eggcase) Silk Protein[J]. Biochemistry, 2005, 44(22): 8006-8012. doi: 10.1021/bi050366u
HU X Y, KOHLER K, FALICK A M, et al. Spider Egg Case Core Fibers: Trimeric Complexes Assembled from TuSp1, ECP-1, and ECP-2[J]. Biochemistry, 2006, 45(11): 3506-3516. doi: 10.1021/bi052105q
BAO R Y, ZHANG H Y, TANG Y F. Biomimetic Synthesis of Natural Products: A Journey to Learn, to Mimic, and to Be Better[J]. Accounts of Chemical Research, 2021, 54(19): 3720-3733. doi: 10.1021/acs.accounts.1c00459
EOM K H, BAEK S, KIM I. N-Heterocyclic Carbene-Catalyzed Random Copolymerization of N-Carboxyanhydrides of A-Amino Acids[J]. Polymers, 2021, 13(21): 3674. doi: 10.3390/polym13213674
FUKUSHIMA Y. Secondary Structural Analysis in the Solid State for Analogous Sequential Polypeptides of Glycine-Rich Sequence of Spider Dragline Silk[J]. Polymer Bulletin, 2000, 45(3): 237-244. doi: 10.1007/s002890070026
DOU Y Y, WANG Z P, HE W Q, et al. Artificial Spider Silk from Ion-Doped and Twisted Core-Sheath Hydrogel Fibres[J]. Nature Communications, 2019, 10(1): 5293. doi: 10.1038/s41467-019-13257-4
HE W Q, WANG M L, MEI G K, et al. Establishing Superfine Nanofibrils for Robust Polyelectrolyte Artificial Spider Silk and Powerful Artificial Muscles[J]. Nature Communications, 2024, 15(1): 3485. doi: 10.1038/s41467-024-47796-2
LEWIS R V, HINMAN M, KOTHAKOTA S, et al. Expression and Purification of a Spider Silk Protein: A New Strategy for Producing Repetitive Proteins[J]. Protein Expression and Purification, 1996, 7(4): 400-406. doi: 10.1006/prep.1996.0060
FAHNESTOCK S R, IRWIN S L. Synthetic Spider Dragline Silk Proteins and Their Production in Escherichia coli[J]. Applied Microbiology and Biotechnology, 1997, 47(1): 23-32. doi: 10.1007/s002530050883
XIA X X, QIAN Z G, KI C S, et al. Native-Sized Recombinant Spider Silk Protein Produced in Metabolically Engineered Escherichia Coli Results in a Strong Fiber[J]. PNAS, 2010, 107(32): 14059-14063. doi: 10.1073/pnas.1003366107
FOONG C P, HIGUCHI-TAKEUCHI M, MALAY A D, et al. A Marine Photosynthetic Microbial Cell Factory as a Platform for Spider Silk Production[J]. Communications Biology, 2020(3): 357.
JIN Q, PAN F, HU C F, et al. Secretory Production of Spider Silk Proteins in Metabolically Engineered Corynebacterium glutamicum for Spinning into Tough Fibers[J]. Metabolic Engineering, 2022, 70: 102-114. doi: 10.1016/j.ymben.2022.01.009
HAUPTMANN V, WEICHERT N, MENZEL M, et al. Native-Sized Spider Silk Proteins Synthesized in Planta via Intein-Based Multimerization[J]. Transgenic Research, 2013, 22(2): 369-377. doi: 10.1007/s11248-012-9655-6
BROOKS A E, STRICKER S M, JOSHI S B, et al. Properties of Synthetic Spider Silk Fibers Based on Argiope Aurantia MaSp2[J]. Biomacromolecules, 2008, 9(6): 1506-1510. doi: 10.1021/bm701124p
SCHILLBERG S, RAVEN N, SPIEGEL H, et al. Critical Analysis of the Commercial Potential of Plants for the Production of Recombinant Proteins[J]. Frontiers in Plant Science, 2019(10): 720.
XU H T, FAN B L, YU S Y, et al. Construct Synthetic Gene Encoding Artificial Spider Dragline Silk Protein and Its Expression in Milk of Transgenic Mice[J]. Animal Biotechnology, 2007, 18(1): 1-12. doi: 10.1080/10495390601091024
LI H, CHEN S N, PIAO S H, et al. Production of Artificial Synthetic Spidroin Gene 4S-Transgenic Cloned Sheep Embryos Using Somatic Cell Nuclear Transfer[J]. Animal Biotechnology, 2021, 32(5): 616-626. doi: 10.1080/10495398.2020.1737098
ANDERSSON M, JOHANSSON J, RISING A. Silk Spinning in Silkworms and Spiders[J]. International Journal of Molecular Sciences, 2016, 17(8): 1290. doi: 10.3390/ijms17081290
FU C J, SHAO Z Z, FRITZ V. Animal Silks: Their Structures, Properties and Artificial Production[J]. Chemical Communications, 2009(43): 6515-6529. doi: 10.1039/b911049f
ZABELINA V, KLYMENKO V, TAMURA T, et al. Genome Engineering and Parthenocloning in the Silkworm, Bombyx mori[J]. Journal of Biosciences, 2015, 40(3): 645-655. doi: 10.1007/s12038-015-9548-y
WEN H X, LAN X Q, ZHANG Y S, et al. Transgenic Silkworms (Bombyx mori) Produce Recombinant Spider Dragline Silk in Cocoons[J]. Molecular Biology Reports, 2010, 37(4): 1815-1821. doi: 10.1007/s11033-009-9615-2
KUWANA Y, SEZUTSU H, NAKAJIMA K I, et al. High-Toughness Silk Produced by a Transgenic Silkworm Expressing Spider (Araneus ventricosus) Dragline Silk Protein[J]. PLoS One, 2014, 9(8): e105325. doi: 10.1371/journal.pone.0105325
YOU Z Y, YE X G, YE L P, et al. Extraordinary Mechanical Properties of Composite Silk through Hereditable Transgenic Silkworm Expressing Recombinant Major Ampullate Spidroin[J]. Scientific Reports, 2018(8): 15956.
TANG X L, YE X G, WANG X X, et al. High Mechanical Property Silk Produced by Transgenic Silkworms Expressing the Spidroins PySp1 and ASG1[J]. Scientific Reports, 2021(11): 20980.
XU J, DONG Q L, YU Y, et al. Mass Spider Silk Production through Targeted Gene Replacement in Bombyx mori[J]. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115(35): 8757-8762.
ZHANG X L, XIA L J, DAY B A, et al. CRISPR/Cas9 Initiated Transgenic Silkworms as a Natural Spinner of Spider Silk[J]. Biomacromolecules, 2019, 20(6): 2252-2264. doi: 10.1021/acs.biomac.9b00193
YU Y, CHEN K, WANG J X, et al. Custom-Designed, Mass Silk Production in Genetically Engineered Silkworms[J]. PNAS Nexus, 2024, 3(4): 128. doi: 10.1093/pnasnexus/pgae128
BOWEN C, DAI B, SARGENT C J, et al. Recombinant Spidroins Fully Replicate Primary Mechanical Properties of Natural Spider Silk[J]. Biomacromolecules, 2018, 19(9): 3853-3860. doi: 10.1021/acs.biomac.8b00980
BHATTACHARYYA G, OLIVEIRA P, KRISHNAJI S T, et al. Large Scale Production of Synthetic Spider Silk Proteins in Escherichia coli[J]. Protein Expression and Purification, 2021, 183: 105839. doi: 10.1016/j.pep.2021.105839
PODDAR H, BREITLING R, TAKANO E. Towards Engineering and Production of Artificial Spider Silk Using Tools of Synthetic Biology[J]. Engineering Biology, 2020, 4(1): 1-6. doi: 10.1049/enb.2019.0017
WERTEN M W T, EGGINK G, COHEN STUART M A, et al. Production of Protein-Based Polymers in Pichia pastoris[J]. Biotechnology Advances, 2019, 37(5): 642-666. doi: 10.1016/j.biotechadv.2019.03.012
WEICHERT N, HAUPTMANN V, HELMOLD C, et al. Seed-Specific Expression of Spider Silk Protein Multimers Causes Long-Term Stability[J]. Frontiers in Plant Science, 2016(7): 6.
HUGIE M R. Expression Systems for Synthetic Spider Silk Protein Production[D]. Logan: Utah State University, 2019.
DECKER R E. Production and Biocompatibility of Spider Silk Proteins in Goat Milk[D]. Logan: Utah State University, 2018.
XU M, JIANG Y K, PRADHAN S, et al. Use of Silk Proteins to Form Organic, Flexible, Degradable Biosensors for Metabolite Monitoring[J]. Frontiers in Materials, 2019(6): 331.
SALEHI S, KOECK K, SCHEIBEL T. Spider Silk for Tissue Engineering Applications[J]. Molecules, 2020, 25(3): 737. doi: 10.3390/molecules25030737
BAKHSHANDEH B, NATEGHI S S, GAZANI M M, et al. A Review on Advances in the Applications of Spider Silk in Biomedical Issues[J]. International Journal of Biological Macromolecules, 2021, 192: 258-271. doi: 10.1016/j.ijbiomac.2021.09.201
KUHBIER J W, REIMERS K, KASPER C, et al. FirstInvestigation of Spider Silk as a Braided Microsurgical Suture[J]. Journal of Biomedical Materials Research Part B, Applied Biomaterials, 2011, 97(2): 381-387.
SPIEß K, WOHLRAB S, SCHEIBEL T. Structural Characterization and Functionalization of Engineered Spider Silk Films[J]. Soft Matter, 2010, 6(17): 4168-4174. doi: 10.1039/b927267d
SLOTTA U, RAMMENSEE S, GORB S, et al. An Engineered Spider Silk Protein Forms Microspheres[J]. Angewandte Chemie International Edition, 2008, 47(24): 4592-4594. doi: 10.1002/anie.200800683
LEAL-EGAÑA A, LANG G, MAUERER C, et al. Interactions of Fibroblasts with Different Morphologies Made of an Engineered Spider Silk Protein[J]. Advanced Engineering Materials, 2012, 14(3): B67-B75.
AIGNER T B, DESIMONE E, SCHEIBEL T. Biomedical Applications of Recombinant Silk-Based Materials[J]. Advanced Materials, 2018, 30(19): 1704636. doi: 10.1002/adma.201704636
GUSTAFSSON L, TASIOPOULOS C P, JANSSON R, et al. Recombinant Spider Silk Forms Tough and Elastic Nanomembranes that are Protein-Permeable and Support Cell Attachment and Growth[J]. Advanced Functional Materials, 2020, 30(40): 2002982. doi: 10.1002/adfm.202002982
TASIOPOULOS C P, GUSTAFSSON L, VAN DER WIJNGAART W, et al. Fibrillar Nanomembranes of Recombinant Spider Silk Protein Support Cell Co-Culture in an in Vitro Blood Vessel Wall Model[J]. ACS Biomaterials Science & Engineering, 2021, 7(7): 3332-3339.
CURRIE H A, DESCHAUME O, NAIK R R, et al. Genetically Engineered Chimeric Silk-Silver Binding Proteins[J]. Advanced Functional Materials, 2011, 21(15): 2889-2895. doi: 10.1002/adfm.201100249
ZHAO L, CHEN D L, YAO Q H, et al. Studies on the Use of Recombinant Spider Silk Protein/Polyvinyl Alcohol Electrospinning Membrane as Wound Dressing[J]. International Journal of Nanomedicine, 2017, 12: 8103-8114. doi: 10.2147/IJN.S47256
JONES J A, HARRIS T I, TUCKER C L, et al. More than Just Fibers: An Aqueous Method for the Production of Innovative Recombinant Spider Silk Protein Materials[J]. Biomacromolecules, 2015, 16(4): 1418-1425. doi: 10.1021/acs.biomac.5b00226
HOLLAND C, NUMATA K, RNJAK-KOVACINA J, et al. The Biomedical Use of Silk: Past, Present, Future[J]. Advanced Healthcare Materials, 2019, 8(1): 1800465. doi: 10.1002/adhm.201800465
GOMES S C, LEONOR I B, MANO J F, et al. Antimicrobial Functionalized Genetically Engineered Spider Silk[J]. Biomaterials, 2011, 32(18): 4255-4266. doi: 10.1016/j.biomaterials.2011.02.040
TROSSMANN V T, SCHEIBEL T. Design of Recombinant Spider Silk Proteins for Cell Type Specific Binding[J]. Advanced Healthcare Materials, 2023, 12(9): 2202660. doi: 10.1002/adhm.202202660
CHOUHAN D, DAS P, THATIKONDA N, et al. Silkworm Silk Matrices Coated with Functionalized Spider Silk Accelerate Healing of Diabetic Wounds[J]. ACS Biomaterials Science & Engineering, 2019, 5(7): 3537-3548.
EL-SHERBINY I M, YACOUB M H. Hydrogel Scaffolds for Tissue Engineering: Progress and Challenges[J]. Global Cardiology Science & Practice, 2013, 2013(3): 316-342.
WIDHE M, JOHANSSON U, HILLERDAHL C O, et al. Recombinant Spider Silk with Cell Binding Motifs for Specific Adherence of Cells[J]. Biomaterials, 2013, 34(33): 8223-8234. doi: 10.1016/j.biomaterials.2013.07.058
SCHILLER T, SCHEIBEL T. Bioinspired and Biomimetic Protein-Based Fibers and Their Applications[J]. Communications Materials, 2024(5): 56.
TUCKER C L, JONES J A, BRINGHURST H N, et al. Mechanical and Physical Properties of Recombinant Spider Silk Films Using Organic and Aqueous Solvents[J]. Biomacromolecules, 2014, 15(8): 3158-3170. doi: 10.1021/bm5007823
HARDY J G, LEAL-EGAÑA A, SCHEIBEL T R. Engineered Spider Silk Protein-Based Composites for Drug Delivery[J]. Macromolecular Bioscience, 2013, 13(10): 1431-1437. doi: 10.1002/mabi.201300233
YOSHIMIZU H, ASAKURA T. Preparation and Characterization of Silk Fibroin Powder and Its Application to Enzyme Immobilization[J]. Journal of Applied Polymer Science, 1990, 40(1-2): 127-134. doi: 10.1002/app.1990.070400111
HARDY J G, SCHEIBEL T R. Production and Processing of Spider Silk Proteins[J]. Journal of Polymer Science Part A: Polymer Chemistry, 2009, 47(16): 3957-3963. doi: 10.1002/pola.23484
LAMMEL A, SCHWAB M, HOFER M, et al. RecombinantSpider Silk Particles as Drug Delivery Vehicles[J]. Biomaterials, 2011, 32(8): 2233-2240. doi: 10.1016/j.biomaterials.2010.11.060
JASTRZEBSKA K, KUCHARCZYK K, FLORCZAK A, et al. Silk as anInnovative Biomaterial for Cancer Therapy[J]. Reports of Practical Oncology and Radiotherapy, 2015, 20(2): 87-98. doi: 10.1016/j.rpor.2014.11.010
GU Y Q, YU L Z, MOU J G, et al. Mechanical Properties and Application Analysis of Spider Silk Bionic Material[J]. e-Polymers, 2020, 20(1): 443-457. doi: 10.1515/epoly-2020-0049
DEPTUCH T, PENDERECKA K, KACZMAREK M, et al. In Vivo Study of the Immune Response to Bioengineered Spider Silk Spheres[J]. Scientific Reports, 2022, 12: 13480. doi: 10.1038/s41598-022-17637-7
ORNITHOPOULOU E, ÅSTRAND C, GUSTAFSSON L, et al. Self-Assembly of RGD-Functionalized Recombinant Spider Silk Protein into Microspheres in Physiological Buffer and in the Presence of Hyaluronic Acid[J]. ACS Applied Bio Materials, 2023, 6(9): 3696-3705. doi: 10.1021/acsabm.3c00373
LU H X, HOSHIBA T, KAWAZOE N, et al. Comparison of Decellularization Techniques for Preparation of Extracellular Matrix Scaffolds Derived from Three-Dimensional Cell Culture[J]. Journal of Biomedical Materials Research Part A, 2012, 100 (9): 2507-2516.
BRANKOVIĆ M, ZIVIC F, GRUJOVIC N, et al. Review of Spider Silk Applications in Biomedical and Tissue Engineering[J]. Biomimetics, 2024, 9(3): 169. doi: 10.3390/biomimetics9030169
LENTZ S, TROSSMANN V T, SCHEIBEL T. Selective Topography Directed Cell Adhesion on Spider Silk Surfaces[J]. Advanced Materials Interfaces, 2023, 10(5): 2201936. doi: 10.1002/admi.202201936
AGAPOV I I, PUSTOVALOVA O L, MOISENOVICH M M, et al. Three-Dimensional Scaffold Made from Recombinant Spider Silk Protein for Tissue Engineering[J]. Doklady Biochemistry and Biophysics, 2009, 426(1): 127-130. doi: 10.1134/S1607672909030016
DING Z Z, WANG Y Y, CHEN F F, et al. Biomimetic Vascular Grafts with Circumferentially and Axially Oriented Microporous Structures for Native Blood Vessel Regeneration[J]. Advanced Functional Materials, 2024, 34(1): 2308888. doi: 10.1002/adfm.202308888
SOFFER L, WANG X Y, ZHANG X H, et al. Silk-Based Electrospun Tubular Scaffolds for Tissue-Engineered Vascular Grafts[J]. Journal of Biomaterials Science, Polymer Edition, 2008, 19(5): 653-664. doi: 10.1163/156856208784089607
HUMENIK M, PAWAR K, SCHEIBEL T. Nanostructured, Self-Assembled Spider Silk Materials for Biomedical Applications[J]. Advances in Experimental Medicine and Biology, 2019, 1174: 187-221.
CHOUHAN D, THATIKONDA N, NILEBÄCK L, et al. Recombinant Spider Silk Functionalized Silkworm Silk Matrices as Potential Bioactive Wound Dressings and Skin Grafts[J]. ACS Applied Materials & Interfaces, 2018, 10(28): 23560-23572.
LIU X F, MILLER A L Ⅱ, PARK S, et al. Two-Dimensional Black Phosphorus and Graphene Oxide Nanosheets Synergistically Enhance Cell Proliferation and Osteogenesis on 3D Printed Scaffolds[J]. ACS Applied Materials & Interfaces, 2019, 11(26): 23558-23572.
HENNECKE K, REDEKER J, KUHBIER J W, et al. Bundles of Spider Silk, Braided into Sutures, Resist Basic Cyclic Tests: Potential Use for Flexor Tendon Repair[J]. PLoS One, 2013, 8(4): e61100. doi: 10.1371/journal.pone.0061100
VASITA R, KATTI D S. Nanofibers and Their Applications in Tissue Engineering[J]. International Journal of Nanomedicine, 2006, 1(1): 15-30. doi: 10.2147/nano.2006.1.1.15
FUKUNISHI T, BEST C A, SUGIURA T, et al. PreclinicalStudy of Patient-Specific Cell-Free Nanofiber Tissue-Engineered Vascular Grafts Using 3-Dimensional Printing in a Sheep Model[J]. The Journal of Thoracic and Cardiovascular Surgery, 2017, 153(4): 924-932. doi: 10.1016/j.jtcvs.2016.10.066
SEIDLITS S K, LEE J Y, SCHMIDT C E. Nanostructured Scaffolds for Neural Applications[J]. Nanomedicine, 2008, 3(2): 183-199. doi: 10.2217/17435889.3.2.183
HERMANSON K D, HUEMMERICH D, SCHEIBEL T, et al. Engineered Microcapsules Fabricated from Reconstituted Spider Silk[J]. Advanced Materials, 2007, 19(14): 1810-1815. doi: 10.1002/adma.200602709
QI Z Z, TAO X S, TAN G, et al. Electro-Responsive Silk Fibroin Microneedles for Controlled Release of Insulin[J]. International Journal of Biological Macromolecules, 2023, 242: 124684. doi: 10.1016/j.ijbiomac.2023.124684
NUMATA K, KAPLAN D L. Silk-Based Gene Carriers with Cell Membrane Destabilizing Peptides[J]. Biomacromolecules, 2010, 11(11): 3189-3195. doi: 10.1021/bm101055m
WOHLRAB S, MVLLER S, SCHMIDT A, et al. Cell Adhesion and Proliferation on RGD-Modified Recombinant Spider Silk Proteins[J]. Biomaterials, 2012, 33(28): 6650-6659. doi: 10.1016/j.biomaterials.2012.05.069
KONWARH R, GUPTA P, MANDAL B B. Silk-Microfluidics for Advanced Biotechnological Applications: A Progressive Review[J]. Biotechnology Advances, 2016, 34(5): 845-858. doi: 10.1016/j.biotechadv.2016.05.001
HUMENIK M, SMITH A M, SCHEIBEL T. Recombinant Spider Silks—Biopolymers with Potential for Future Applications[J]. Polymers, 2011, 3(1): 640-661. doi: 10.3390/polym3010640