On Optimization Screening Conditions of α-Amylase Inhibitor by Central Composite Design Coupled with Response Surface Methodology

YIN Yan-xia , ZHU Qi-feng , LIU Han-jie , TIAN Jin-hong

doi:10.13718/j.cnki.xsxb.2015.04.017

Journal of Southwest China Normal University(Natural Science Edition)

Search Advanced

Message Board

Dear readers, authors and reviewers,you can add a message on this page. We will reply to you as soon as possible!

Name
	Name cannot be empty!
E-mail
	Mailbox cannot be empty! Mailbox cannot be empty!
Telephone
	Mobile number cannot be empty! Please enter a valid mobile number!
Title

Content
Verification Code

PDF
Cite
Share

facebook

twitter

google

linkedin

2015 Volume 40 Issue 4

Article Contents

Previous Article Next Article

YIN Yan-xia , ZHU Qi-feng , LIU Han-jie , TIAN Jin-hong. On Optimization Screening Conditions of α-Amylase Inhibitor by Central Composite Design Coupled with Response Surface Methodology[J]. Journal of Southwest China Normal University(Natural Science Edition), 2015, 40(4). doi: 10.13718/j.cnki.xsxb.2015.04.017

Citation:

YIN Yan-xia , ZHU Qi-feng , LIU Han-jie , TIAN Jin-hong. On Optimization Screening Conditions of α-Amylase Inhibitor by Central Composite Design Coupled with Response Surface Methodology[J]. Journal of Southwest China Normal University(Natural Science Edition), 2015, 40(4). doi: 10.13718/j.cnki.xsxb.2015.04.017

On Optimization Screening Conditions of α-Amylase Inhibitor by Central Composite Design Coupled with Response Surface Methodology

YIN Yan-xia , ZHU Qi-feng , LIU Han-jie , TIAN Jin-hong

More Information

Abstract

Central Composite Design (CCD) coupled with Response Surface Method (RSM ) has been per‐formed to optimize α‐amylase inhibitor screening conditions .On the basis of single factor experiments , four factors includes starch solution concentration ,heat preservation temperature ,dosage of DNS ,boiling time that affect α‐amylase activity have been optimized .Results show as follow s :α‐amylase concentration 1mg/mL ,starch solution concentration 17 mg/mL ,preservation temperature 46 ℃ ,holding time 15min , dosage of DNS 2 mL ,boiling time 6 min .The result is stable and reliable and can be used for α‐amylase in‐hibitor screening .
- α-amylase inhibitor，central composite design，response surface method,
References
Access History

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Export Citation

PDF

XML

Article Metrics

Article views(1608) PDF downloads(0) Cited by(0)

Access History