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2018 Volume 43 Issue 5
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LIU Fang-jun, GONG Cai-hui, LIU Sha. On Role of Alveolar Epithelial Cells in Hyperoxic Acute Lung Injury[J]. Journal of Southwest China Normal University(Natural Science Edition), 2018, 43(5): 95-100. doi: 10.13718/j.cnki.xsxb.2018.05.016
Citation: LIU Fang-jun, GONG Cai-hui, LIU Sha. On Role of Alveolar Epithelial Cells in Hyperoxic Acute Lung Injury[J]. Journal of Southwest China Normal University(Natural Science Edition), 2018, 43(5): 95-100. doi: 10.13718/j.cnki.xsxb.2018.05.016
shu

On Role of Alveolar Epithelial Cells in Hyperoxic Acute Lung Injury

  • Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing Key Laboratory of Pediatrics, China International Science and Technology Cooperation base for Child Development and Critical Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing 400014, China

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  • Received Date: 02/03/2016
  • Observed the expressions of thyroid transcription factor-1(TTF-1)and surfactant protein B (SP-B) in the lungs of newborn mice with exposed to hyperoxia in order to explain the possible pathogenesis of hyperoxia acute lung injury(HALI). Totally 30 postnatal day 1 mice, which were randomly divided into hyperoxia exposed group and air control group(n=15)respectively. hyperoxia exposed for 72 hours induce the HALI.assessed the wet/dry weight(W/D)ratio,observed the changes of lung pathology, injury score andradical alveolar counts. The protein and mRNA expressions of thyroid transcription factor-1(TTF-1)and surfactant protein B (SP-B) were detected by immunofluorescence and real-time PCR after 24,48and72 hours, respectively. Contrasted the air control group, lung tissue gradually showed increased interstitial cell and pulmonary edema at hyperoxia exposed for 48 hours.andW/D ratio and injury scores of 48 hours(all PP<0.05). The mRNA and protein expression of TTF-1 and SP-B in lung tissue were decreased markedly at 72 hours of hyperoxia exposed group compared with the air control group. In newborn mice hyperoxia exposed can induced lung injury, and disrupts pulmonary epithelial may be plays an very important role in the development of HALI.
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On Role of Alveolar Epithelial Cells in Hyperoxic Acute Lung Injury

  • Received Date: 02/03/2016

Abstract: Observed the expressions of thyroid transcription factor-1(TTF-1)and surfactant protein B (SP-B) in the lungs of newborn mice with exposed to hyperoxia in order to explain the possible pathogenesis of hyperoxia acute lung injury(HALI). Totally 30 postnatal day 1 mice, which were randomly divided into hyperoxia exposed group and air control group(n=15)respectively. hyperoxia exposed for 72 hours induce the HALI.assessed the wet/dry weight(W/D)ratio,observed the changes of lung pathology, injury score andradical alveolar counts. The protein and mRNA expressions of thyroid transcription factor-1(TTF-1)and surfactant protein B (SP-B) were detected by immunofluorescence and real-time PCR after 24,48and72 hours, respectively. Contrasted the air control group, lung tissue gradually showed increased interstitial cell and pulmonary edema at hyperoxia exposed for 48 hours.andW/D ratio and injury scores of 48 hours(all PP<0.05). The mRNA and protein expression of TTF-1 and SP-B in lung tissue were decreased markedly at 72 hours of hyperoxia exposed group compared with the air control group. In newborn mice hyperoxia exposed can induced lung injury, and disrupts pulmonary epithelial may be plays an very important role in the development of HALI.

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