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2021 Volume 34 Issue 1
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LIU Lie-hua, DONG Peng, KUANG Mi, et al. Diversity Analysis of Soil Microbial Community in the Rhizosphere Soil of Bacterial Wilt-Diseased and Healthy Pepper Plants[J]. PLANT HEALTH AND MEDICINE, 2021, (1): 41-47. doi: 10.13718/j.cnki.zwys.2021.01.008
Citation: LIU Lie-hua, DONG Peng, KUANG Mi, et al. Diversity Analysis of Soil Microbial Community in the Rhizosphere Soil of Bacterial Wilt-Diseased and Healthy Pepper Plants[J]. PLANT HEALTH AND MEDICINE, 2021, (1): 41-47. doi: 10.13718/j.cnki.zwys.2021.01.008
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Diversity Analysis of Soil Microbial Community in the Rhizosphere Soil of Bacterial Wilt-Diseased and Healthy Pepper Plants

  • 1. School of Plant Protection, Southwest University, Chongqing 400715, China;

  • 2. Chongqing General Station of Agricultural Technology Extension, Chongqing 401121, China;

  • 3. Chongqing Anbang Agricultural Development Co., Ltd, Chongqing 400030, China

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  • Received Date: 16/12/2020

  • MSC: S436.418.1

  • In order to provide a theoretical basis for the green control of pepper bacterial wilt, a study was made in which the characteristics of soil microbial community structure of pepper bacterial wilt-affected and healthy pepper plants in the areas where this disease frequently occurs were systematically analyzed. Based on the 16S rDNA gene high-throughput sequencing technology, the structure and composition of the soil microbial community in the rhizosphere of pepper bacterial wilt-diseased and healthy plants were compared, and the biology eco plate culture technology was used to study the characteristics of metabolic diversity and function diversityof the soil microbial community. A significant difference was detected between diseased plants and healthy plants in the composition of rhizosphere soil microbial community, the OTU (operational taxonomic unit) of the soil affected by pepper bacterial wilt being 4 566, and that of the healthy soil being 4 167. According to the bacterial species information of OTU, the soil bacterial community structure was analyzed. Proteobacteria were found to be the dominant bacterial groups in diseased and healthy soil, followed by Actinomycetes. Among them, the relative abundance of Gemmatimonadetes and Armatimonadetes in the rhizosphere soil of healthy plants was 4.37 and 3.87 times higher than that of diseased plants, respectively, while the relative abundance of Firmicutes was 3.87 times higher in the rhizosphere soil of diseased plants than that of healthy plants. Significant differences also existed in the diversity of soil microbial metabolism between pepper bacterial wilt-affected soil and healthy soil. At the same time, the metabolism of the microbial community in healthy soil was found to be significantly enhanced, especially the use of phenolic compounds increased significantly, which hada significant impact on disease resistance of pepper plants.
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Diversity Analysis of Soil Microbial Community in the Rhizosphere Soil of Bacterial Wilt-Diseased and Healthy Pepper Plants

  • Received Date: 16/12/2020

Abstract: In order to provide a theoretical basis for the green control of pepper bacterial wilt, a study was made in which the characteristics of soil microbial community structure of pepper bacterial wilt-affected and healthy pepper plants in the areas where this disease frequently occurs were systematically analyzed. Based on the 16S rDNA gene high-throughput sequencing technology, the structure and composition of the soil microbial community in the rhizosphere of pepper bacterial wilt-diseased and healthy plants were compared, and the biology eco plate culture technology was used to study the characteristics of metabolic diversity and function diversityof the soil microbial community. A significant difference was detected between diseased plants and healthy plants in the composition of rhizosphere soil microbial community, the OTU (operational taxonomic unit) of the soil affected by pepper bacterial wilt being 4 566, and that of the healthy soil being 4 167. According to the bacterial species information of OTU, the soil bacterial community structure was analyzed. Proteobacteria were found to be the dominant bacterial groups in diseased and healthy soil, followed by Actinomycetes. Among them, the relative abundance of Gemmatimonadetes and Armatimonadetes in the rhizosphere soil of healthy plants was 4.37 and 3.87 times higher than that of diseased plants, respectively, while the relative abundance of Firmicutes was 3.87 times higher in the rhizosphere soil of diseased plants than that of healthy plants. Significant differences also existed in the diversity of soil microbial metabolism between pepper bacterial wilt-affected soil and healthy soil. At the same time, the metabolism of the microbial community in healthy soil was found to be significantly enhanced, especially the use of phenolic compounds increased significantly, which hada significant impact on disease resistance of pepper plants.

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