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2023 Volume 2 Issue 6
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WU Xiaojun, WANG Hancheng, LIN Musen, et al. Effect of Difenoconazole on the Diversity of Phyllosphere Microbial Communities of Leaf Infected with Tobacco Taget Spot[J]. PLANT HEALTH AND MEDICINE, 2023, (6): 1-12. doi: 10.13718/j.cnki.zwyx.2023.06.001
Citation: WU Xiaojun, WANG Hancheng, LIN Musen, et al. Effect of Difenoconazole on the Diversity of Phyllosphere Microbial Communities of Leaf Infected with Tobacco Taget Spot[J]. PLANT HEALTH AND MEDICINE, 2023, (6): 1-12. doi: 10.13718/j.cnki.zwyx.2023.06.001
shu

Effect of Difenoconazole on the Diversity of Phyllosphere Microbial Communities of Leaf Infected with Tobacco Taget Spot

  • 1. College of Tobacco, Guizhou University, Guiyang 550025, China;

  • 2. Guizhou Academy of Tobacco Science, Guiyang 550081, China;

  • 3. Daozhen Municipal Company of Guizhou Tobacco Company, Daozhen Guizhou 563500, China;

  • 4. College of Life Science, Yangtze University, Jingzhou Hubei 434025, China

More Information
  • Received Date: 20/11/2023

  • MSC: S154.3；S572

  • In order to investigate the changing patterns of the phyllosphere microbial community of tobacco infected with tobacco target spot after difenoconazole treatment, this paper analyzed the community structure and diversity of phyllosphere microbial communities in diseased and healthy tobacco leaves at different periods after difenoconazole treatment by using high-throughput sequencing technology. The results showed that the number of dominant genera of fungi and bacterium increased in the diseased and healthy tobacco leaves compared to those before treatment. The dominant genera of fungi were distributed in Basidiomycota and Ascomycota, and the dominant genera of bacterium were distributed in Proteobacteria. The relative abundance of dominant genera of fungi and bacterium in the diseased and healthy tobacco leaves differed at different time points from 1 to 18 days after difenoconazole (90 g/hm2) treatment, but the overall trend was consistent. In the fungal community, the relative abundance was in a decreasing-increasing-decreasing trend for Thanatephorus, an increasing-decreasing trend for Plectosphaerella, Sampaiozyma, Symmetrospora, Pleurotus and Alternaria, and a decreasing trend for Cercospora. In the bacterial community, the relative abundance was in an increasing-decreasing-increasing-decreasing trend for Pseudomonas, Sphingomonas and Curtobacterium, and an increasing-decreasing trend for Pantoea and Methylobacterium. The diversity and richness of the phyllosphere fungal and bacterial communities of diseased and healthy tobacco leaves changed synchronously, with an overall increasing-decreasing trend, and the differences in the diversity and richness of the phyllosphere fungal communities of diseased and healthy tobacco gradually decreased. In summary, the overall changes in the structure and diversity of the phyllosphere fungal and bacterial communities in diseased and healthy tobacco were similar after difenoconazole treatment, and the differences in the phyllosphere fungal and bacterial communities between diseased and healthy tobacco decreased with the extension of time.
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  • [1] 邱梦娟, 黎妍妍, 徐婷婷, 等. 湖北省烟草靶斑病病原鉴定及其菌丝融合群遗传分化研究[J]. 中国烟草科学, 2022, 43(5):50-55.

    Google Scholar

    [2] 苏燕妮, 董雪, 赵艳琴, 等. 东北地区烟草靶斑病菌(Rhizoctonia solani)融合群、致病力分化及品种抗病性研究[J]. 植物保护, 2016, 42(1):170-174.

    Google Scholar

    [3] 孙宏宇. 烟草靶斑病发生及防治技术研究进展[J]. 中国植保导刊, 2015, 35(7):23-26.

    Google Scholar

    [4] 彭友兵. 烟草靶斑病的绿色防控策略及建议[J]. 植物医生, 2020, 33(6):34-36.

    Google Scholar

    [5] 常哈拿, 占浩鑫, 张琳, 等.东北地区人参生炭疽菌对苯醚甲环唑和戊唑醇的敏感性及田间抗性监测[J].植物病理学报, 2023, 19(5):1-15.

    Google Scholar

    [6] 喻敏, 杨艳, 李文, 等. 苯醚甲环唑复配型杀虫剂研究进展[J]. 安徽农学通报, 2023, 29(2):94-96.

    Google Scholar

    [7] 尹秀娟, 肖艳松, 李思军, 等. 防治烟草靶斑病复配增效配方筛选及田间防效[J]. 农药, 2022, 61(6):453-457.

    Google Scholar

    [8] 孙美丽, 汪汉成, 郭沫言, 等. 4种杀菌剂对烟草靶斑病菌的抑菌活性[J]. 贵州农业科学, 2022, 50(9):62-68.

    Google Scholar

    [9] 孙美丽, 史彩华, 肖本青, 等. 烟草靶斑病叶际微生物群落结构与多样性分析[J]. 烟草科技, 2023, 56(4):1-9.

    Google Scholar

    [10] DAI Y F, WU X M, WANG H C, et al. Spatio-Temporal Variation in the Phyllospheric Microbial Biodiversity of Alternaria alternata-Infected Tobacco Foliage [J]. Frontiers in Microbiology, 2022, 13:920109.

    Google Scholar

    [11] CHEN X, WICAKSONO W A, BERGG, et al. Bacterial Communities in the Plant Phyllosphere Harbour Distinct Responders to a Broad-Spectrum Pesticide [J]. Science of the Total Environment, 2021, 751:141799.

    Google Scholar

    [12] SUN M L, WANG H C, SHI C H, et al. Effect of Azoxystrobin on Tobacco Leaf Microbial Composition and Diversity [J]. Frontiers in Plant Science, 2023, 13:1101039.

    Google Scholar

    [13] 杨宽, 王慧玲, 叶坤浩, 等. 叶际微生物及与植物互作的研究进展[J]. 云南农业大学学报(自然科学), 2021, 36(1):155-164.

    Google Scholar

    [14] 付刘元, 陈金鹏, 王栓, 等. 禾谷镰孢菌对苯醚甲环唑的敏感性基线及与其他杀菌剂敏感性的相关性分析[J]. 农药学学报, 2021, 23(4):694-702.

    Google Scholar

    [15] 刘宇星, 董醇波, 邵秋雨, 等. 叶际微生物与植物健康研究进展[J]. 微生物学杂志, 2022, 42(2):88-98.

    Google Scholar

    [16] 李娜. 乌头叶面微生物区系与乌头霜霉病的相关性研究[D]. 成都:成都中医药大学, 2017.

    Google Scholar

    [17] 刘亭亭, 汪汉成, 孙美丽, 等. 波尔多液对烟草叶际微生物群落结构与代谢功能的影响[J]. 农药学学报, 2022, 24(6):1446-1455.

    Google Scholar

    [18] RASTOGI G, SBODIO A, TECH J J, et al. Leaf Microbiota in an Agroecosystem:Spatiotemporal Variation in Bacterial Community Composition on Field-Grown Lettuce [J]. The ISME Journal, 2012, 6(10):1812-1822.

    Google Scholar

    [19] INNEREBNER G, KNIEF C, VORHOLT J A. Protection of Arabidopsis Thaliana AgainstLeaf-Pathogenic Pseudomonas Syringae by Sphingomonas Strains in a Controlled Model System [J]. Applied and Environmental Microbiology, 2011, 77(10):3202-3210.

    Google Scholar

    [20] 刘畅, 汪汉成, 谢红炼, 等. 感染赤星病烟草叶际细菌的多样性分析[J]. 烟草科技, 2020, 53(2):8-14.

    Google Scholar

    [21] 刘亭亭, 曾陨涛, 汪汉成, 等. 赤星病发生期不同成熟度烟叶叶际微生物代谢与群落结构[J]. 中国烟草科学, 2021, 42(6):22-29.

    Google Scholar

    [22] CHEN Q L, CAI L, WANG H C, et al. Fungal Composition and Diversity of the Tobacco Leaf Phyllosphere during Curing of Leaves [J]. Frontiers in Microbiology, 2020, 11:554051.

    Google Scholar

    [23] 邱薇, 刘磊, 康杰, 等. 假单胞菌属双组分信号转导系统调控吩嗪生物合成研究进展[J]. 微生物学通报, 2022, 49(1):352-362.

    Google Scholar

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Effect of Difenoconazole on the Diversity of Phyllosphere Microbial Communities of Leaf Infected with Tobacco Taget Spot

  • Received Date: 20/11/2023

Abstract: In order to investigate the changing patterns of the phyllosphere microbial community of tobacco infected with tobacco target spot after difenoconazole treatment, this paper analyzed the community structure and diversity of phyllosphere microbial communities in diseased and healthy tobacco leaves at different periods after difenoconazole treatment by using high-throughput sequencing technology. The results showed that the number of dominant genera of fungi and bacterium increased in the diseased and healthy tobacco leaves compared to those before treatment. The dominant genera of fungi were distributed in Basidiomycota and Ascomycota, and the dominant genera of bacterium were distributed in Proteobacteria. The relative abundance of dominant genera of fungi and bacterium in the diseased and healthy tobacco leaves differed at different time points from 1 to 18 days after difenoconazole (90 g/hm2) treatment, but the overall trend was consistent. In the fungal community, the relative abundance was in a decreasing-increasing-decreasing trend for Thanatephorus, an increasing-decreasing trend for Plectosphaerella, Sampaiozyma, Symmetrospora, Pleurotus and Alternaria, and a decreasing trend for Cercospora. In the bacterial community, the relative abundance was in an increasing-decreasing-increasing-decreasing trend for Pseudomonas, Sphingomonas and Curtobacterium, and an increasing-decreasing trend for Pantoea and Methylobacterium. The diversity and richness of the phyllosphere fungal and bacterial communities of diseased and healthy tobacco leaves changed synchronously, with an overall increasing-decreasing trend, and the differences in the diversity and richness of the phyllosphere fungal communities of diseased and healthy tobacco gradually decreased. In summary, the overall changes in the structure and diversity of the phyllosphere fungal and bacterial communities in diseased and healthy tobacco were similar after difenoconazole treatment, and the differences in the phyllosphere fungal and bacterial communities between diseased and healthy tobacco decreased with the extension of time.

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