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2022 Volume 1 Issue 6
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TIAN Minghui, TENG Kai, TIAN Feng, et al. Analysis of Bacterial Community Structure Characteristics in Rhizosphere Soil of Different Tobacco Bacterial Wilt Resistant and Susceptible Varieties[J]. PLANT HEALTH AND MEDICINE, 2022, (6): 42-49. doi: 10.13718/j.cnki.zwyx.2022.06.005
Citation: TIAN Minghui, TENG Kai, TIAN Feng, et al. Analysis of Bacterial Community Structure Characteristics in Rhizosphere Soil of Different Tobacco Bacterial Wilt Resistant and Susceptible Varieties[J]. PLANT HEALTH AND MEDICINE, 2022, (6): 42-49. doi: 10.13718/j.cnki.zwyx.2022.06.005
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Analysis of Bacterial Community Structure Characteristics in Rhizosphere Soil of Different Tobacco Bacterial Wilt Resistant and Susceptible Varieties

  • 1. Xiangxi Branch of Hunan Tobacco Company, Xiangxi Hunan 416000, China;

  • 2. College of Plant Protection, Southwest University, Chongqing 400715, China

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  • Received Date: 10/08/2022

  • MSC: S572

  • In this study, field experiments were conducted in Xiangxi, Hunan Province, using tobacco varieties 'Yunyan 87''HN2146''K326' and 'GZ36' with different resistance to tobacco bacterial wilt as materials. 16S rDNA was used to analyze the characteristics of rhizosphere soil bacterial community structure of four varieties by high-throughput sequencing method. The results showed that there were significant differences in microbial community structure between susceptible variety 'Yunyan 87' and resistant varieties 'GZ36' and 'HN2146'. The rhizosphere of susceptible variety 'Yunyan 87' was mainly dominated by Proteobacteria, while resistant varieties 'K326' and 'HN2146' were mainly dominated by Acidobacteriota and Gemmatimonadota. In resistant varieties 'K326' and 'HN2146', the relative abundances of disease resistant bacterial groups such as Gemmatimonadota were 5.43% and 4.80%, respectively, which were significantly higher than that of 'Yunyan 87' (4.16%). In addition, the relative abundance of Ralstonia in resistant varieties 'GZ36' 'K326' and 'HN2146' was 0.002%, 0.009% and 0.005%, respectively, which was significantly lower than 0.12% of 'Yunyan 87'. It shows that the roots of disease resistant varieties can enrich beneficial microbial populations, inhibit the population abundance of Ralstonia, and improve their own disease resistance. This study analyzed the differences of rhizosphere soil bacterial community structure of different tobacco bacterial wilt resistant and susceptible varieties, and provided theoretical support for the microecological mechanism of tobacco plant resistance to bacterial wilt, and the prevention and control of bacterial wilt in the future.
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Analysis of Bacterial Community Structure Characteristics in Rhizosphere Soil of Different Tobacco Bacterial Wilt Resistant and Susceptible Varieties

  • Received Date: 10/08/2022

Abstract: In this study, field experiments were conducted in Xiangxi, Hunan Province, using tobacco varieties 'Yunyan 87''HN2146''K326' and 'GZ36' with different resistance to tobacco bacterial wilt as materials. 16S rDNA was used to analyze the characteristics of rhizosphere soil bacterial community structure of four varieties by high-throughput sequencing method. The results showed that there were significant differences in microbial community structure between susceptible variety 'Yunyan 87' and resistant varieties 'GZ36' and 'HN2146'. The rhizosphere of susceptible variety 'Yunyan 87' was mainly dominated by Proteobacteria, while resistant varieties 'K326' and 'HN2146' were mainly dominated by Acidobacteriota and Gemmatimonadota. In resistant varieties 'K326' and 'HN2146', the relative abundances of disease resistant bacterial groups such as Gemmatimonadota were 5.43% and 4.80%, respectively, which were significantly higher than that of 'Yunyan 87' (4.16%). In addition, the relative abundance of Ralstonia in resistant varieties 'GZ36' 'K326' and 'HN2146' was 0.002%, 0.009% and 0.005%, respectively, which was significantly lower than 0.12% of 'Yunyan 87'. It shows that the roots of disease resistant varieties can enrich beneficial microbial populations, inhibit the population abundance of Ralstonia, and improve their own disease resistance. This study analyzed the differences of rhizosphere soil bacterial community structure of different tobacco bacterial wilt resistant and susceptible varieties, and provided theoretical support for the microecological mechanism of tobacco plant resistance to bacterial wilt, and the prevention and control of bacterial wilt in the future.

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