基于微卫星标记的西方蜜蜂抗螨蜂种群体遗传多态性分析
Genetic Diversity Analysis of Mite-Resistance Honeybees (Apis mellifera) Based on Microsatellite Markers
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摘要: 为了分析西方蜜蜂抗螨蜂种的群体遗传多态性情况, 采用18对微卫星(SSR)引物对抗螨蜂种子一代和子二代, 并与同场饲养不抗螨的本地意蜂和浙大蜜浆高产蜂种进行比对, 共计22个群体进行遗传多样性检测. 结果表明: 遗传一致度在0.2时, 抗螨蜂种子代发生一定的分化, 其他两个品系基本一致. 抗螨蜂种子代之间的亲缘关系较近(遗传距离在0.043 9以内), 与本地意蜂和浙大蜜浆高产蜂种各自相互分开. 从而对抗螨蜂种的选育和保护具有重要的指导意义.Abstract: Varroa-resistant honeybees of A.mellifera L.has a strong resistance to Varroa destructo, which were named as F1 and F2.Firstly the genetic diversity of F1 and F2 was investigated with 18 microsatellite markers.Secondly, the mite-resistance bees of the local A.mellifera (Yunnan, Southwest China) and the high yield bees of royal jelly breed from Zhejiang University (East China) were chosen for comparison analysis.The results show, in 22 sample colonies, when Nei's genetic identity index exceeded 0.2, distinct genetic divergence was found filial generation of mite-resistance bees, while other breeds were the same nearly.The F1 and F2 of mite-resistance bees had a close descent relationship (the genetic distances between them were 0.0439), with the local A.mellifera and the high yield bees of royal jelly breed from Zhejiang University separated from each other.
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