Composition and Diversity of Phyllospheric Bacterial Community in Tobacco Leaves Infected by Tobacco Weather Fleck
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摘要: 气候斑点病是一种为害烟草的重要非侵染性病害，为解析烟草气候斑点病叶际细菌群落结构和多样性，利用lllumina高通量测序技术对不同时期感病与健康烟叶叶际微生物群落组成进行测序分析.结果表明，感病烟叶叶际细菌优势菌属为微小杆菌属(Exiguobacterium)、假单胞菌属(Pseudomonas)、泛菌属(Pantoea)和不动杆菌属(Acinetobacter).健康烟叶的优势细菌属为假单胞菌属(Pseudomonas)、微小杆菌属(Exiguobacterium)、不动杆菌属(Acinetobacter)、泛菌属(Pantoea)和肠球菌属(Enterococcus).发病前期，感病烟叶叶际细菌的多样性和丰富度均高于健康烟叶，而发病中后期，感病烟叶叶际细菌的多样性和丰富度均低于健康烟叶.其中，发病烟叶叶际细菌属unclassified_f__Enterobacteriaceae和微杆菌属(Microbacterium)的相对丰度显著高于健康烟叶，健康烟叶的unclassified_f__Comamonadaceae、norank_f__norank_o__Chloroplast、不黏柄菌属(Asticcacaulis)、贪铜菌属(Cupriavidus)、norank_f__Microscillaceae、norank_f__Mitochondria和科里氏杆菌属(Cloacibacterium)的相对丰度显著高于发病烟叶.结果表明，烟草气候斑点病和健康烟叶叶际细菌群落结构、多样性、优势菌属及相对丰度均存在一定差异.Abstract: Climatic spot disease is an important non-invasive disease affecting tobacco, in order to analyze the structure and diversity of the interleaf bacterial communities of tobacco climatic spot disease, the composition of the interleaf microbial communities of infected and healthy tobacco leaves at different times was sequenced and analyzed by using Illumina high-throughput sequencing technology. The results showed that the dominant bacterial genera in diseased tobacco were Exiguobacterium, Pseudomonas, Pantoea, and Acinetobacter. The dominant bacterial genera in healthy tobacco were Pseudomonas, Exiguobacterium, Acinetobacter, Pantoea and Enterococcus. In the early stage of disease infection, the diversity and abundance of interleaf bacteria were higher than that of healthy tobacco, while in the middle and late stage of disease infection, the diversity and abundance of interleaf bacteria were lower than that of healthy tobacco. Among them, the relative abundance of interleaf bacterial genera unclassified__f__Enterobacteriaceae and Microbacterium in diseased tobacco was significantly higher than that of healthy tobacco. unclassified__f__Comamonadaceae, norank__f__norank__o___Chloroplast, unclassified_f__Asticcacaulis, Cupriavidus, norank__f__Microscillaceae, norank__f__Mitochondria, and Cloacibacterium of healthy tobacco were significantly higher than those of diseased tobacco leaves. This indicates that there are some differences in the structure, diversity, dominant genera and relative abundance of interleaf bacterial communities between tobacco climacteric mottle diseased leaves and healthy tobacco leaves.
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Key words:
- tobacco weather fleck /
- phyllospheric bacterium /
- high-throughput sequencing /
- community structure /
- diversity .
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