Analysis of the Microecological Factors of Target Leaf Spot of Tobacco Variety 'K326' in Chongqing Tobacco Area
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摘要: 为明确烟草靶斑病在重庆烟区“K326”烟草品种上对叶际微生物群落结构的影响，在重庆市酉阳县分别采集移栽40 d、60 d、80 d和100 d后具有烟草靶斑病与健康的烟叶样品，采用高通量测序方法分析样品中细菌群落结构，并结合烟株生长期气象因子和病害发生情况进行分析.移栽后40 d靶斑病开始发生，且发病率和病情指数呈上升趋势，移栽后60～80 d为发病高峰期.发病叶中叶际细菌优势菌门为变形菌门和厚壁菌门，优势菌属为假单胞菌属、微小杆菌属等.健康叶叶际细菌的优势菌门分别为蓝藻门、放线菌门、拟杆菌门等，优势菌属为不动杆菌属、微小杆菌属、芽孢杆菌属、泛细菌属等.健康叶的整体群落丰度大于发病叶，移栽40 d、60 d和100 d后采集样品的发病叶与健康叶叶际细菌群落结构比较差异具有统计学意义.靶斑病发生较重情况下，发病叶与健康叶叶际细菌群落结构上差异具有统计学意义，提示靶斑病的发生影响了叶际细菌群落结构.Abstract: In order to determine the key factors and microbial structure of the leaf microenvironment of tobacco target spot disease in 'K326' tobacco variety in Chongqing tobacco region, 'K326' tobacco variety was taken as experimental materials. Samples of diseased and healthy leaves of tobacco plants were collected in Youyang County, Chongqing at 40 d, 60 d, 80 d and 100 d after transplanting. The bacterial community structure in the leaves was analyzed by high-throughput sequencing method, and the climatic factors and disease occurrence during the growth period of tobacco plants were analyzed. At 40 days after transplantation, the onset of target spot disease was observed, exhibiting an upward trend in both disease incidence and severity index, and the period between 60 and 80 days after transplantation was identified as the peak phase of disease incidence. The dominant bacteria in the diseased leaf boundary were Proteobacteria and Thick-walled Bacteria. The dominant bacteria were Panbacterial, Pseudomonas, Microbacterium, etc. The dominant phyla of the healthy leaf boundary bacteria were:Thickwallida, Cyanophyta, Actinomyceta, Bacteroides, etc. The dominant genera were Pseudomonas, Acinetobacter, Microbacterium, Bacillus, Pantobacterium etc. The overall community richness of healthy leaves was greater than that of diseased leaves. The bacterial community structure of diseased leaves and healthy leaves collected on the 40, 60 and 100 days after transplanting was significant different. In the case of severe target spot disease, there were significant differences in the bacterial community structure between the diseased leaves and the healthy leaves.
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Key words:
- tobacco target spot /
- foliar microorganisms /
- high throughput sequencing /
- community structure .
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[1] 周建全, 张忠光, 董雪, 等. 不同药剂对烟草靶斑病的抑菌作用及田间药效研究[J]. 安徽农业科学, 2015, 43(25):96-97, 99. [2] 陈丹阳, 甘勇, 张万金等. 烟草靶斑病拮抗菌的筛选鉴定及抗逆性研究[J/OL]. 四川农业大学学报, 1-13[2024-02-27]. https://doi.org/10.16036/j.issn.1000-2650.202108044. [3] 刘斯泓, 纪明山. 防治烟草靶斑病的复配药剂配方筛选及田间药效试验[J]. 江苏农业科学, 2014, 42(6):140-143. [4] 侯慧慧, 孙剑萍, 刘子仪, 等. 云南烟草靶斑病(Rhizoctonia solani Kühn)病原鉴定及其融合群研究[J]. 沈阳农业大学学报, 2018, 49(2):203-208. [5] XIA B, XU C T, XU J K, et al. First Report of Target Leaf Spot on Flue-Cured Tobacco by Rhizoctonia Solani AG-3 in Sichuan, China[J]. Plant Disease, 2019, 103(3):581. [6] 徐传涛, 张崇, 张明金, 等. 四川省烟草靶斑病病原鉴定及生物防治研究[J]. 湖北农业科学, 2021, 60(8):87-90. [7] LINDOW S E, BRANDL M T. Microbiology of the Phyllosphere[J]. Applied and Environmental Microbiology, 2003, 69(4):1875-1883. [8] 刘天波, 滕凯, 周向平, 等. 拮抗菌群对烟草野火病的防治效果及叶际微生物群落多样性的影响[J]. 微生物学通报, 2021, 48(8):2643-2652. [9] 刘宇星, 董醇波, 邵秋雨, 等. 叶际微生物与植物健康研究进展[J]. 微生物学杂志, 2022, 42(2):88-98. [10] VORHOLT J A. Microbial Life in the Phyllosphere[J]. Nature Reviews Microbiology, 2012, 10:828-840. [11] 刘亭亭, 曾陨涛, 汪汉成, 等. 赤星病发生期不同成熟度烟叶叶际微生物代谢与群落结构[J]. 中国烟草科学, 2021, 42(6):22-29. [12] 罗路云, 张卓, 金德才, 等. 南瓜白粉病不同病情等级下叶际细菌群落结构和多样性[J]. 植物病理学报, 2017, 47(5):688-695. [13] 杨超. 重庆烟区主要生态因子特征及其对烤烟产质量的影响[D]. 重庆:西南大学, 2015. [14] LI PD, ZHU ZR, ZHANG Y, et al. The Phyllosphere Microbiome Shifts Toward Combating Melanose Pathogen[J]. Microbiome, 2022, 10(1):1-17. [15] 刘畅, 汪汉成, 谢红炼, 等. 感赤星病烟叶的真菌群落结构分析[J]. 贵州农业科学, 2019, 47(7):54-59. [16] 孙美丽, 史彩华, 肖本青, 等. 烟草靶斑病叶际微生物群落结构与多样性分析[J]. 烟草科技, 2023, 56(4):1-9. [17] 刘畅, 汪汉成, 谢红炼, 等. 感染赤星病烟草叶际细菌的多样性分析[J]. 烟草科技, 2020, 53(2):8-14. [18] 吴小军, 汪汉成, 孙美丽, 等. 烟草角斑病叶际微生物群落结构与多样性分析[J]. 烟草科技, 56(10):1-10. [19] 黄宇, 汪汉成, 陈乾丽, 等. 感染白粉病烟株典型病级叶际真菌群落结构与多样性分析[J]. 烟草科技, 2021, 54(4):8-14. -
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