翘鳞肉齿菌多糖的分离纯化及抗氧化活性的研究
- 西华师范大学生命科学学院/西南野生动植物资源保护教育部重点实验室,四川南充,637009
摘要: 为了分离纯化翘鳞肉齿菌多糖，解析其结构特征，并研究其抗氧化活性．运用DEAE‐cellulose column 进行柱层析分离纯化翘鳞肉齿菌多糖，高效凝胶渗透色谱（HPGPC）方法研究其均重分子量，通过红外光谱解析其结构特征，进一步研究其对羟基自由基（?OH）、1，1二苯基2苦基肼自由基（DPPH－）和2，2联氮二（3乙基苯并噻唑6磺酸）二铵盐自由基（ABTS＋）的清除作用，同时研究了翘鳞肉齿菌多糖在 H2 O2作用下对PC12细胞的保护能力．其结果显示从四川省小金县翘鳞肉齿菌子实体分离纯化得到一种水溶性杂多糖（SIKP‐1）纯品，其重均分量约为2.0×104Da ．通过化学方法研究了不同质量浓度的翘鳞肉齿菌多糖（SIKP‐1）抗氧化活性，结果表明：当SIKP‐1质量浓度为0.1 mg／mL 时，其对 DPPH －自由基的清除率可达到40.63％；当 SIKP‐1质量浓度达到0.32 mg／mL时，对?OH自由基的清除率可达到51.61％；当SIKP‐1的质量浓度为3 mg／mL时，对ABTS＋自由基的清除率可达69.75％；在细胞生物学实验中，结果显示在用终质量浓度为0.5，1，2 mg／mL的SIKP‐1处理下， PC12能免于 H2 O2的损伤，随着剂量的增加其存活率越高，分别为15.89％，27.60％，29.36％．综上试验结果显示，翘鳞肉齿菌多糖（SIKP‐1）具有显著的抗氧化功能，因此可以作为一种理想的抗氧化剂资源．
On Extraction,Purificationand Antioxidant Activity of Polysaccharidefrom Sarcodon imbricatum (L .:Fr) Karst .
- 西华师范大学生命科学学院/西南野生动植物资源保护教育部重点实验室,四川南充,637009
Keywords:
- 翘鳞肉齿菌 /
- 多糖 /
- 分离纯化 /
- 抗氧化活性
Abstract: Toseparate and purificate the Polysaccharide from Sarcodon imbricatum (L .:Fr) Karst .,the au‐thor has analyzed its structure characteristics and studied their antioxidant activity .By means of hot water for extraction ,of DEAE‐cellulose column chromatography for separation and purification ,of gel perme‐ation chromatography (HPGPC) method to study the average molecular weight ,andof infrared spectrum analysis for the structure characteristics ,further research on hydroxyl free radical (OH) ,1 ,1‐two phen‐yl‐2‐bitter hydrazyl free radical (DPPH -) and 2 ,2‐nitrogen‐two (3‐ethyl benzene and thiophene‐6‐sulfonic acid) two ammonium salt free radical (ABTS+ ) scavenging effect of the polysaccharide ,at the same time study the protection ability of the polysaccharide on PC12 cells in the presence of H2 O2 .A water soluble polysaccharide (SIKP‐1) was isolated and purified from Sarcodon imbricatum (L .:Fr) Karst .,the aver‐age weight is about 2 × 104 Da .The antioxidant activity of polysaccharide (SIKP‐1) with different concen‐trations was studied by different chemical method ,the results showed that when the SIKP‐1 concentration was 0 .1 mg/ml ,the DPPH‐free radical scavenging rate can reach 40 .63% ;when SIKP‐1 concentration reached 0 .32 mg/ml ,the OH free radical scavenging rate can reach 51 .61% ;when the SIKP‐1 concentra‐tion was 3 mg /ml ,the ABTS+ free radical scavenging rate was 69 .75% ;in the cell biology experiment , results show that the final concentration of SIKP‐1 were 0 .5 ,1 ,2 mg/mL ,the PC12 cell can avoid the in‐jury of H2 O2 ,with the survival rate15 .89% ,27 .60% ,29 .36% ,respectively .The results show that the polysaccharide (SIKP‐1) has significant antioxidant activity ,which can be used as an ideal natural antioxi‐dant resource in medicine and human health .
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