Toxicity Evaluation of Different Fungicides to the Pathogen of Osmanthus Gray Spot
-
摘要: 为筛选有效防治桂花灰斑病的高效低毒的杀菌剂,为该病的防控提供研究基础,本研究采用菌丝生长速率法分别测定6种化学农药和5种生物农药对桂花灰斑病病原菌的室内毒力.结果表明,11种药剂对桂花灰斑病病原菌生长的抑制率明显不同,但化学农药抑菌效果普遍比生物农药好.其中,在化学农药中,10%苯醚甲环唑和500 g/L异菌脲悬浮剂抑菌效果表现最好,EC50值分别为0.33μg/mL和0.52μg/mL; 50%氯溴异氰尿酸素抑菌效果最差,EC50仅为117.54μg/mL.在生物农药中,3%四霉素的抑菌效果较好,EC50值为40.83μg/mL;其次是100亿芽孢/克枯草芽孢杆菌,EC50值为63.73μg/mL;抑菌效果最差的是3%中生菌素,EC50为1 341.60μg/mL.在农业生产中,化学农药可选用10%苯醚甲环唑、 500 g/L异菌脲悬浮剂、 430 g/L戊唑醇、 300 g/L苯甲·丙环唑进行防治,能达到快速有效的防治效果;生物农药则可选用3%四霉素、 100亿芽孢/g枯草芽孢杆菌来进行防治.Abstract: To screen effective and low-toxic fungicides for the prevention and control of gray spot disease of Osmanthus fragrans, and provide research basis for the prevention and control of the disease, the indoor toxicity of 6 chemical pesticides and 5 biological pesticides to the pathogen of Pestalotiopsis scirrofaciens was determined by the mycelial growth rate method. The results showed thatthe inhibitory rates of 11 kinds of pesticides on the growth of the pathogen of Pestalotiopsis scirrofaciens quite different, but the inhibitory effects of chemical pesticides were generally better than the biological pesticides. Among the chemical pesticides, 10% difenoconazole and 500 g/L Isocarburon suspension had the best bacteriostatic effect, with EC50 values of 0.33 μg/mL and 0.52 μg/mL, respectively. The bacteriostatic effect of 50% Chlorobromoisocyanuratewas the worst, EC50was only 117.54 μg/mL.Among biopesticides, 3% tetracycline had a good bacteriostatic effect, with an EC50 value of 40.83 μg/mL. The second was 10 billion spores/gram of Bacillus subtilis, with an EC50 value of 63.73 μg/mL. The worst bacteriostatic effect was 3% Mesobiotin, the EC50was 1,341.60 μg/mL.In agricultural production, 10% difenoconazole, 500 g/L Isocarburon suspension agent, 430 g/L tebuconazole and 300 g/L Benzofeniconazole can be chosen as chemical pesticides to achieve rapid and effective control effect. Biological pesticides control can use 3% tetracycline and 10 billion spores/gram of Bacillus subtilis.
-
Key words:
- Osmanthus /
- gray spot disease /
- fungicides /
- indoor toxicity determination .
-
-
[1] 顾恒,李玲,欧阳绮霞,等.盐胁迫对3个桂花品种生长和生理特性的影响 [J].中国野生植物资源,2020,39(10):28-34. [2] 杨玉婷,黄淑容,龙仪珂,等.桂花病虫害综合防治研究进展 [J].热带农业科学,2022,42(3):73-82. [3] 孟婷婷,齐鹰博,刘闯,等.树莓类拟盘多毛孢叶斑病病原菌的鉴定 [J].沈阳农业大学学报,2020,51(1):111-116. [4] 张传飞,戚佩坤.广东省人心果病原真菌研究 [J].华南农业大学学报,1994,15(4):31-36. [5] 徐永辉,邹燕敏,蔡平,等.桂花拟盘多毛孢灰斑病生物学特性研究 [J].上海农业科技,2008(4):86-87. [6] 金亚杰.闽楠主要病害病原鉴定及其生物学特性测定和防治药剂筛选 [D].福州:福建农林大学,2017. [7] 张晓勇,李树江,王亮,等.山茶灰斑病病原菌鉴定及防治药剂初步筛选 [J].植物保护,2019,45(4):209-215,242. [8] 任海英,戚行江,陈安良,等.十种杀菌剂对杨梅凋萎病的药效评价 [J].果树学报,2013,30(5):848-853. [9] PAN X L,DONG F S,WU X H,et al.Progress of the Discovery,Application,and Control Technologies of Chemical Pesticides in China [J].Journal of Integrative Agriculture,2019,18(4):840-853. [10] 朱权辉.生物农药开发与运用的现状及其在西藏的前景和可行性初探 [J].河南农业,2021(23):42-43. [11] 周蒙.中国生物农药发展的现实挑战与对策分析 [J].中国生物防治学报,2021,37(1):184-192. [12] 杨帅,徐淑兵,金岩,等.5种生物农药对芦笋茎枯病的防治效果 [J].中国蔬菜,2021(7):83-87. [13] 黄大野,万中义,朱志刚,等.诺沃霉素A对茶轮斑病的防治效果 [J].植物保护,2019,45(2):243-246. [14] 邱德文.生物农药的发展现状与趋势分析 [J].中国生物防治学报,2015,31(5):679-684. [15] 王岩,彭强,赵小明,等.生物降解农药残留的研究进展 [J].中国农学通报,2021,37(18):117-124. -
计量
- 文章访问数: 474
- HTML全文浏览数: 455
- PDF下载数: 165
- 施引文献: 0