氮源对复合菌系 XCP-1降解氨化稻秆性能的影响
摘要: 利用复合菌系降解稻秆生产生物基产品是生物质资源化利用的重要方式.在这一过程中,氮源是影响微生物增殖和降解效率的重要因素.研究利用中温复合菌系 XCP‐1(筛选于新疆多年连作棉田土壤)降解氨化稻秆,分析有机氮源对复合菌系 XCP‐1发酵过程中微生物增殖,NH3‐N 与 pH 动态变化,氮源利用效率以及稻秆降解效率的影响.结果表明,添加豆粕和玉米酒糟能促进复合菌系 XCP‐1的增殖和稻秆降解率的提高.复合菌系 XCP‐1对豆粕中氮的利用率高于玉米酒糟,总氮利用率达到78.1%;对氨化稻秆的降解率也高于添加玉米酒糟,总降解率达到68.3%,纤维素、半纤维素和木质素降解率分别达到78.4%,85.6%和14.3%.所添加氮源(豆粕和玉米酒糟)对发酵过程中的 pH 值影响较小,pH 值始终维持在5.0~9.5之间.以廉价豆粕或玉米酒糟代替蛋白胨作为稻秆生物降解的工业氮源,降低了稻秆生物转化的成本,这为稻秆转化为生物基产品的工业化应用提供了理论基础.
Effect of Nitrogen Sources on Biodegradation of Ammoniated Rice Straw by Microbial Consortium XCP-1
Abstract: Biodegradation of rice straw using microbial consortium is an important way for conversion of bi‐omass into useful products .In the process of biodegradation ,nitrogen source is a main factor influencing the microbial grow th and digest efficiency .The mesophilic microbial consortium XCP‐1 (isolated from soil of continuous cropping cotton field in Xinjiang ) was employed to digest ammoniated straw .This study seeks to investigate the influence of organic nitrogen source on the microbial growth ,changes of NH3‐N and pH ,and biodegradation of rice straw .Results showed that organic nitrogen (corn lees and soybean meal) could increase microbial grow th and digestion of rice straw .It was further indicated that total nitro‐gen utilization rate of soybean meal (78.1% ) was higher than that of corn lees (41.2% ) .Moreover ,the degradation rate of ammoniated rice straw supplied with soybean meal was also higher than that of corn lees ,and degradation rate of cellulose ,hemicellulose ,lignin and total ammoniated rice straw were 78.4% , 85.6% ,14.3% and 68.3% ,respectively .While the nitrogen resource (corn lees or soybean meal) has lit‐tle effect on pH ,which was ranging from 5.0 to 9.5 .By adding economical nitrogen source (corn lees or soybean meal) ,biodegradation costs of rice straw can be reduced .It’s important for the industrialization production of biomass‐based products .