纤维素降解真菌的分离筛选
Isolation and Screening of Cellulose-degradation Fungus
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摘要: 采用涂布平板法和刚果红平板透明圈法，从常年堆积腐烂落叶下的腐殖土壤中分离获得11株有一定纤维素降解活性的真菌菌株．采用滤纸失重法和3，5二硝基水杨酸比色法，进一步测定了11株真菌对纤维素的降解能力，结果表明：编号为 LY4‐2，LY4‐4，LY4‐5和 LY5‐1的4株菌株对纤维素表现出不同程度的降解活性，其中 LY4‐5菌株表现出较好的降解活性，滤纸失重率为44.96％，羧甲基纤维素钠酶活为41.25 IU ，滤纸酶活为27.63 IU ．Abstract: Eleven fungi strains with a certain activity of cellulose‐degradation were isolated from the humus soil under pile‐up rotting leaves all the year round by using spread plate method and transparent circle of Congo red plate method .By the filter paper weight‐loss method and 3 ,5‐Dinitrosalicylic acid colorimetric method ,cellulose‐degradation activity of the 11 fungi strains was studied .The result showed that four strains numbered LY4‐2 ,LY4‐4 ,LY4‐5 and LY5‐1 had different cellulose‐degradation activity .LY4‐5 stain had the best activity , the filter paper weight‐loss rate was 44.96% ,CMCase activity was 41.25 IU , FPAase activity was 27.63 IU .
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