梨ISSR-PCR反应体系的正交优化研究

桂腾琴,孙敏,陆春连,刘群,代婷,柏和燕

doi:10.13718/j.cnki.xsxb.2014.12.008

西南师范大学学报(自然科学版)

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梨ISSR-PCR反应体系的正交优化研究

桂腾琴,孙敏,陆春连,刘群,代婷,柏和燕. 梨ISSR-PCR反应体系的正交优化研究[J]. 西南师范大学学报(自然科学版), 2014, 39(12). doi: 10.13718/j.cnki.xsxb.2014.12.008

引用本文:

桂腾琴,孙敏,陆春连,刘群,代婷,柏和燕. 梨ISSR-PCR反应体系的正交优化研究[J]. 西南师范大学学报(自然科学版), 2014, 39(12). doi: 10.13718/j.cnki.xsxb.2014.12.008

GUI Teng-qin,SUN Min,LU Chun-lian,LIU Qun,DAI Ting,BO He-yan. On Optimization of Reaction System in Pear-Using Orthogonal Design[J]. Journal of Southwest China Normal University(Natural Science Edition), 2014, 39(12). doi: 10.13718/j.cnki.xsxb.2014.12.008

Citation:

GUI Teng-qin,SUN Min,LU Chun-lian,LIU Qun,DAI Ting,BO He-yan. On Optimization of Reaction System in Pear-Using Orthogonal Design[J]. Journal of Southwest China Normal University(Natural Science Edition), 2014, 39(12). doi: 10.13718/j.cnki.xsxb.2014.12.008

梨ISSR-PCR反应体系的正交优化研究

桂腾琴,孙敏,陆春连,刘群,代婷,柏和燕

兴义民族师范学院生物与化学学院,贵州兴义562400; 西南大学生命科学学院,重庆400715; 贵州省民族药用生物资源研究与开发重点实验室,贵州兴义562400 ; 西南大学生命科学学院,重庆,400715 ; 兴义民族师范学院生物与化学学院,贵州兴义,562400

On Optimization of Reaction System in Pear-Using Orthogonal Design

GUI Teng-qin,SUN Min,LU Chun-lian,LIU Qun,DAI Ting,BO He-yan

摘要: Orthogonal design has been applied to optimize ISSR‐PCR amplification system of in pear in five factors such as primer ,dNTPs ,template DNA ,Taq DNA polymerase and Mg2+ at four levels .The result of PCR has been analyzed by range analysis and variance analysis .An optimal reaction system (25μL) has been established ,i .e .2 .5 μL 10 × Buffer ,2 .0 mmol/L M g2+ ,0 .25 mmol/L dN T Ps ,0 .25 μmol/L prim‐er ,60 ng template DNA ,0 .75 U Taq DNA polymerase .The results indicate that the optimized reaction system was stable and repeatable .The objective of the study is to provide a scientific basis for the con‐structing DNA fingerprints and genetic diversity analysis of in pear .
- inpear,orthogonaldesign,ISSRsystemoptimization
Abstract: 梨；正交设计； ISSR体系优化

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梨ISSR-PCR反应体系的正交优化研究

桂腾琴,孙敏,陆春连,刘群,代婷,柏和燕

兴义民族师范学院生物与化学学院,贵州兴义562400; 西南大学生命科学学院,重庆400715; 贵州省民族药用生物资源研究与开发重点实验室,贵州兴义562400 ; 西南大学生命科学学院,重庆,400715 ; 兴义民族师范学院生物与化学学院,贵州兴义,562400

关键词:

inpear,orthogonaldesign,ISSRsystemoptimization

摘要: Orthogonal design has been applied to optimize ISSR‐PCR amplification system of in pear in five factors such as primer ,dNTPs ,template DNA ,Taq DNA polymerase and Mg2+ at four levels .The result of PCR has been analyzed by range analysis and variance analysis .An optimal reaction system (25μL) has been established ,i .e .2 .5 μL 10 × Buffer ,2 .0 mmol/L M g2+ ,0 .25 mmol/L dN T Ps ,0 .25 μmol/L prim‐er ,60 ng template DNA ,0 .75 U Taq DNA polymerase .The results indicate that the optimized reaction system was stable and repeatable .The objective of the study is to provide a scientific basis for the con‐structing DNA fingerprints and genetic diversity analysis of in pear .