蕹菜过氧化物酶固定化条件分析及固定化对其理化性质影响

王红扬,孙才云,黄忙,唐云明

doi:10.13718/j.cnki.xsxb.2014.12.012

西南师范大学学报(自然科学版)

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蕹菜过氧化物酶固定化条件分析及固定化对其理化性质影响

王红扬,孙才云,黄忙,唐云明. 蕹菜过氧化物酶固定化条件分析及固定化对其理化性质影响[J]. 西南师范大学学报(自然科学版), 2014, 39(12). doi: 10.13718/j.cnki.xsxb.2014.12.012

引用本文:

WANG Hong-yang,SUN Cai-yun,HUANG Mang,TANG Yun-ming. On Conditions of Immobilization and Its Effects on Characterization of Peroxidase from Ipomoea Aquatica Forsk[J]. Journal of Southwest China Normal University(Natural Science Edition), 2014, 39(12). doi: 10.13718/j.cnki.xsxb.2014.12.012

Citation:

WANG Hong-yang,SUN Cai-yun,HUANG Mang,TANG Yun-ming. On Conditions of Immobilization and Its Effects on Characterization of Peroxidase from Ipomoea Aquatica Forsk[J]. Journal of Southwest China Normal University(Natural Science Edition), 2014, 39(12). doi: 10.13718/j.cnki.xsxb.2014.12.012

蕹菜过氧化物酶固定化条件分析及固定化对其理化性质影响

王红扬,孙才云,黄忙,唐云明

西南大学生命科学学院,重庆400715; 重庆市甘薯工程研究中心,重庆400715; 三峡库区生态环境教育部重点实验室,重庆400715

On Conditions of Immobilization and Its Effects on Characterization of Peroxidase from Ipomoea Aquatica Forsk

WANG Hong-yang,SUN Cai-yun,HUANG Mang,TANG Yun-ming

摘要: Electrophoresis‐purity peroxidase (POD) from Ipomoea aquatica Forskhas beenimmobilized with 0 .2% PVA‐CA as carrier ,saturated boric acid solution as cross linker and CaCl2 as fixing agent .Their effects on POD immobilizationare determined by means of single factor analysis ,and the optimal combina‐tion of their agents via orthogonal test are 0 .2% PVA 3% CA ,the 1∶2 ratio between PVA‐VA and en‐zyme ,saturated boric acid 6% CaCl2 and 45min immobilizing time .After immobilization ,the optimal temperature ,stability in high temperature and the reuse stability enhanced .
- IpomoeaaquaticaForsk,peroxidase,immobilization,characterization
Abstract: 固定化；蕹菜；过氧化物酶；理化性质

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蕹菜过氧化物酶固定化条件分析及固定化对其理化性质影响

王红扬,孙才云,黄忙,唐云明

西南大学生命科学学院,重庆400715; 重庆市甘薯工程研究中心,重庆400715; 三峡库区生态环境教育部重点实验室,重庆400715

关键词:

IpomoeaaquaticaForsk,peroxidase,immobilization,characterization

摘要: Electrophoresis‐purity peroxidase (POD) from Ipomoea aquatica Forskhas beenimmobilized with 0 .2% PVA‐CA as carrier ,saturated boric acid solution as cross linker and CaCl2 as fixing agent .Their effects on POD immobilizationare determined by means of single factor analysis ,and the optimal combina‐tion of their agents via orthogonal test are 0 .2% PVA 3% CA ,the 1∶2 ratio between PVA‐VA and en‐zyme ,saturated boric acid 6% CaCl2 and 45min immobilizing time .After immobilization ,the optimal temperature ,stability in high temperature and the reuse stability enhanced .