Synergistic Effect of S-Abscisic Acid Combined with Zhongshengmycin on Control of Tobacco Bacterial Wilt
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摘要: 为明确植物免疫诱抗剂联合生物农药对烟草青枯病的防控可行性和防治效果,以及为缓解烟草青枯病的发生提供实践基础,本研究以"云烟87"为研究对象,研究不同浓度的S-诱抗素诱导烟草对青枯病菌抗性及联合中生菌素对烟草青枯病的田间防效和烤烟农艺性状的影响.室内盆栽结果表明,1.63~26.00 mg/L S-诱抗素诱导抗病性差异较大,其中1.63~6.50 mg/L和26.00 mg/L的S诱抗素有利于青枯病发生,而13.00 mg/L可有效缓解烟草青枯病的发生.田间试验结果表明,S-诱素与中生菌素联用能够促进烤烟生长,其中,株高、茎围、最大叶长和最大叶面积等农艺性状与清水对照比较,差异具有统计学意义.此外,13.00 mg/L S-诱抗素与1.33 mg/L中生菌素联用可有效缓解烟草青枯病的发生,不同时期的增效系数CEC值均表现为相加作用,而且在发病初期(7月8日)和发病后期(8月5日)防治效果分别可达到92.05%,59.87%.因此,田间S-诱抗素与中生菌素联用有望用于缓解烟草青枯病的发生以及提升烟草的农艺性状.Abstract: To clarify the control feasibility and effect of plant immune inducers combined with bio-pesticides against tobacco bacterial wilt (Ralstonia solanacearu), and provide a practical basis for alleviating the occurrence of tobacco bacterial wilt. Taking ‘Yunyan 87’ cultivar as the research object, the effects of different concentrations of S-Abscisic acid on the disease resistance of flue-cured tobacco and the effect of combined with Zhongshengmycin on control of tobacco bacterial wilt in the field and agronomic traits of flue-cured tobacco were studied. The results of indoor pot experiment showed that there were great differences on disease resistance induced by 1.63~26.00 mg/L S-Abscisic acid, among which 1.63~6.50 mg/L and 26.00 mg/L of S-Abscisic acid were beneficial to the occurrence of bacterial wilt, while the 13.00 mg/L of S-Abscisic acid effectively alleviated the occurrence of tobacco bacterial wilt. The results of field experiments showed that the combination of S-Abscisic acid and Zhongshengmycin could promote the growth of flue-cured tobacco. The agronomic characters such as plant height, stem circumference, maximum leaf length and maximum leaf area were significantly higher than those of the clear water control. In addition, the combination of 13.00 mg/L S-Abscisic acid and 1.33 mg/L zhongshengmycin could effectively alleviate the occurrence of tobacco bacterial wilt. In the early stage (July 8th) and late stage (August 5th) of infection, the control effect could reach 92.05% and 59.87%, respectively. Therefore, the combination of S-Abscisic acid and zhongshengmycin will be expected to alleviate the occurrence of tobacco bacterial wilt in the field and improve the agronomic traits of tobacco.
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Key words:
- tobacco bacterial wilt /
- S-Abscisic acid /
- Zhongshengmycin /
- control effect .
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[1] 丁伟, 刘颖, 李石力, 等. 烟草青枯病的病原特性及流行预警[J]. 植物医生, 2020, 33(2):1-10. [2] 何洪令, 李钠钾, 孙成成, 等. 烟草青枯病的生物防治研究进展[J]. 植物医生, 2021, 34(2):4-8. [3] 何明兴, 沈亮, 邱恒良, 等. 烟草青枯病的发生及防治[J]. 现代农业科技, 2019(1):111-112, 115. [4] 王垚, 韩松庭, 杨亮, 等. 生物有机肥对烟草青枯病防控的研究进展[J]. 植物医生, 2020, 33(6):18-23. [5] 解艳玲, 杜军, 沈振荣, 等. S-诱抗素研究进展[J]. 安徽农业科学, 2013, 41(4):1517-1518, 1554. [6] 李晗. 干旱处理下S-诱抗素和黄腐酸对藜麦幼苗生长的影响[D]. 张家口:河北北方学院, 2021. [7] 万宣伍, 李国慧, 邢艳, 等. 10%S-诱抗素可溶性粉剂对葡萄产量及品质的影响[J]. 食品安全质量检测学报, 2021, 12(3):925-930. [8] 苏正川, 谢兴伟, 白伟, 等. S-诱抗素对水稻稻瘟病防治和产量的影响[J]. 农药, 2020, 59(8):601-603, 606. [9] 刘文涛, 熊仁科, 吴红波, 等. S-诱抗素对花生叶斑病防治和产量的影响[J]. 农药科学与管理, 2022, 43(3):34-39. [10] 朱昌雄, 蒋细良, 孙东园, 等. 新农用抗生素——中生菌素[J]. 精细与专用化学品, 2002, 10(16):14-17. [11] 周柳, 潘忠成, 周晶晶, 等. 5%中生菌素可溶性粒剂防治柑橘溃疡病的田间药效试验[J]. 四川农业科技, 2022(5):35-38. [12] 刘洋, 赵文静, 沈斐, 等. 不同农药对桃细菌性穿孔病菌的毒力和田间防效[J]. 现代农药, 2020, 19(5):43-47. [13] 王瑞, 雷霁卿, 查仕连, 等. 二十种生物源杀菌剂对三株猕猴桃溃疡病病原菌的毒力测定[J]. 北方园艺, 2018(21):54-59. [14] 王帅, 刘召阳, 高小宁, 等. 10种生物源杀菌剂对苹果树腐烂病菌的室内活性评价[J]. 西北林学院学报, 2019, 34(1):150-156. [15] 魏晓兵, 傅俊范, 周如军. 14种生物杀菌剂对人参锈腐病菌室内毒力测定[C]. 中国植物保护学会学术年会, 2014. [16] 高娃, 张冬梅, 刘丹, 等. 番茄溃疡病防治药剂的筛选[J]. 安徽农业科学, 2018, 46(20):137-140. [17] 高汝佳. 桃枝枯病生物防治研究[D]. 扬州:扬州大学, 2017. [18] 周开拓, 蒋承耿, 王秋萍, 等. 中生菌素对贵州烟区烟草青枯病的毒力测定及田间防效试验[J]. 农业科技与装备, 2017(11):14-16. [19] 杨亮. 羟基香豆素类化合物对青枯雷尔氏菌致病特性的调控作用研究[D]. 重庆:西南大学, 2020. [20] 王垚, 张宸, 漆夏燕, 等. 高海拔烟区移栽时间和育苗措施对烟草生长发育及经济性状的影响[J]. 植物医生, 2021, 34(5):30-36. [21] 郭庆明, 徐笑宇, 陈永明, 等. 几种诱抗剂诱导烟草抗青枯病效果及其与链霉素混用增效作用[J]. 农药, 2012, 51(8):608-610. [22] 陈绍荣, 龚骁, 王美华. S-诱抗素在大樱桃增产提质中的作用试验简报[J]. 磷肥与复肥, 2017, 32(12):18-19. [23] 温秀萍, 郭至乐. S-诱抗素在吐鲁番结果枣树上的应用[J]. 北方果树, 2010(6):7. [24] 姚晨涛, 姜兴印, 孙晓, 等. 0.25% S-诱抗素水剂调节番茄生长田间药效试验[J]. 现代农药, 2019, 18(2):50-51, 56. [25] 张建业, 杜庆志, 刘翔, 等. S-诱抗素对盐碱地玉米生长及产量的调控作用[J]. 农药, 2021, 60(11):853-858.
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