Virus in Humus of Tobacco Field and Its Transmission Efficiency in Fengjie, Chongqing
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摘要: 明确烟田腐殖质携带病毒种类及其传播效率,对于烟田腐殖质处理防治病毒病传播至关重要.本研究采用RT-PCR技术,检测重庆市奉节地区烟田腐殖质中携带病毒种类,并种植健康无毒苗于腐殖质中,检测烟苗带毒情况,明确腐殖质传毒能力.结果表明,重庆市奉节地区腐殖质中烟草花叶病毒、黄瓜花叶病毒带毒率为100.00%和66.67%,高温待腐熟腐殖质烟草花叶病毒传毒率为66.67%,高温腐熟烟草花叶病毒传毒率为0,高温待腐熟和高温腐熟黄瓜花叶病毒传播率为0.综上所述,本研究首次明确腐殖质能携带烟草花叶病毒和黄瓜花叶病毒,且高温腐熟腐殖质能有效抑制烟草花叶病毒传播,高温能有效抑制黄瓜花叶病毒传播,为烟田腐殖质处理抑制病毒传播提供科学依据.Abstract: To clarify the types of viruses carried by humus in tobacco fields and their transmission efficiency is very important for the treatment of humus in tobacco fields to prevent the spread of viral diseases. In this study, RT-PCR technology was used to detect the types of viruses carried in the humus of tobacco fields in Fengjie, Chongqing. The healthy virus-free seedlings were planted in the humus to detect the infection status of tobacco seedlings and determine the ability of humus to transmit viruses. The results showed that the detection rate of tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) in the humus in tobacco fields of Fengjie, Chongqing was 100.00% and 66.67%, respectively. The detection rate of TMV in the humus pre-compost under high temperature was 66.67%. The transmission rate of TMV in the high-temperature composted humus was 0. The transmission rates of CMV in the humus pre-compost under high-temperature and high-temperature composted humus were zero. In conclusion, this study is the first report to confirm that humus can carry TMV and CMV, and high temperature composted humus can effectively inhibit the spread of TMV, high temperature can effectively inhibit the spread of CMV. The findings of this study provided a scientific basis for humus treatment in tobacco field to inhibit the spread of virus.
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Key words:
- tobacco field /
- humus /
- virus /
- infection efficiency .
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