JING D L, LIU X Y, HE Q, et al. Genome Assembly of Wild Loquat (Eriobotrya japonica) and Resequencing Provide New Insights into the Genomic Evolution and Fruit Domestication in Loquat[J]. Horticulture Research, 2022, 10(2): uhac265.
|
XU H X, MENG D, YANG Q, et al. Sorbitol Induces Flower Bud Formation via the MADS-Box Transcription Factor EjCAL in Loquat[J]. Journal of Integrative Plant Biology, 2023, 65(5): 1241-1261.
|
WANG D, CHEN Q Y, GUO Q G, et al. Metabolomic Analyses Reveal Potential Mechanisms Induced by Melatonin Application for Tolerance of Water Deficit in Loquat (Eriobotrya japonica Lindl. )[J]. Scientia Horticulturae, 2023, 308: 111569.
|
PENG Z, ZHAO C B, LI S Q, et al. Integration of Genomics, Transcriptomics and Metabolomics Identifies Candidate Loci Underlying Fruit Weight in Loquat[J]. Horticulture Research, 2022, 9: uhac037.
|
REN R, GAO J, YIN D M, et al. Highly Efficient Leaf Base Protoplast Isolation and Transient Expression Systems for Orchids and Other Important Monocot Crops[J]. Frontiers in Plant Science, 2021, 12: 626015.
|
STELMACH-WITYK K, SZYMONIK K, GRZEBELUS E, et al. Development of an Optimized Protocol for Protoplast-to-Plant Regeneration of Selected Varieties of Brassica oleracea L[J]. BMC Plant Biology, 2024, 24(1): 1279.
|
WOO J W, KIM J, KWON S I, et al. DNA-Free Genome Editing in Plants with Preassembled CRISPR-Cas9 Ribonucleoproteins[J]. Nature Biotechnology, 2015, 33(11): 1162-1164.
|
LIU H, HU D X, DU P X, et al. Single-Cell RNA-Seq Describes the Transcriptome Landscape and Identifies Critical Transcription Factors in the Leaf Blade of the Allotetraploid Peanut (Arachis hypogaea L. )[J]. Plant Biotechnology Journal, 2021, 19(11): 2261-2276.
|
YOO S D, CHO Y H, SHEEN J. Arabidopsis Mesophyll Protoplasts: A Versatile Cell System for Transient Gene Expression Analysis[J]. Nature Protocols, 2007, 2(7): 1565-1572.
|
CAO J M, YAO D M, LIN F, et al. PEG-Mediated Transient Gene Expression and Silencing System in Maize Mesophyll Protoplasts: A Valuable Tool for Signal Transduction Study in Maize[J]. Acta Physiologiae Plantarum, 2014, 36(5): 1271-1281.
|
HAN S C, QU G, LI X H, et al. Highly Efficient Endosperm and Pericarp Protoplast Preparation System for Transient Transformation of Endosperm-Related Genes in Wheat[J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2023, 155(1): 165-174.
|
ZHANG Y, SU J B, DUAN S, et al. A Highly Efficient Rice Green Tissue Protoplast System for Transient Gene Expression and Studying Light/Chloroplast-Related Processes[J]. Plant Methods, 2011, 7(1): 30.
|
YANG W H, REN J Q, LIU W R, et al. An Efficient Transient Gene Expression System for Protein Subcellular Localization Assay and Genome Editing in Citrus Protoplasts[J]. Horticultural Plant Journal, 2023, 9(3): 425-436.
|
WANG X P, XU L L, LIU X X, et al. Development of Potent Promoters that Drive the Efficient Expression of Genes in Apple Protoplasts[J]. Horticulture Research, 2021, 8(1): 211.
|
LI X Y, SANDGRIND S, MOSS O, et al. Efficient Protoplast Regeneration Protocol and CRISPR/Cas9-Mediated Editing of Glucosinolate Transporter (GTR) Genes in Rapeseed (Brassica napus L. )[J]. Frontiers in Plant Science, 2021, 12: 680859.
|
林顺权, 陈振光. 枇杷原生质体植株再生[J]. 园艺学报, 1996, 23(4): 313-318.
|
林顺权, 陈振光. 山梨醇对枇杷原生质体分离和培养的效应[J]. 福建农业大学学报, 1997, 26(4): 401-406.
|
WANG S, WANG L, LIU Z, et al. An Efficient System for Mesophyll Protoplast Isolation, Purification, and Transformation in Loquat: Studies on Fluorescent Marker Analysis and Subcellular Localization[J]. Horticulturae, 2025, 11: 391.
|
孙鹤, 郎志宏, 朱莉, 等. 玉米、小麦、水稻原生质体制备条件优化[J]. 生物工程学报, 2013, 29(2): 224-234.
|
景欢欢, 李冰清, 牛晓茹, 等. 月季原生质体制备及培养的研究进展[J]. 分子植物育种, 2022, 3: 1-13.
|
李志美, 张碧佩, 伍青, 等. 菊花花瓣原生质体分离与瞬时转化体系的建立[J]. 植物生理学报, 2023, 59(10): 1951-1963.
|
LIN Z, HUANG L J, YU P Y, et al. Development of a Protoplast Isolation System for Functional Gene Expression and Characterization Using Petals of Camellia oleifera[J]. Plant Physiology and Biochemistry, 2023, 201: 107885.
|
SHI D Q, WU J, TANG H B, et al. Single-Pollen-Cell Sequencing for Gamete-Based Phased Diploid Genome Assembly in Plants[J]. Genome Research, 2019, 29(11): 1889-1899.
|
张峰. 马铃薯花粉原生质体的分离与培养[D]. 兰州: 甘肃农业大学, 2001.
|
张盖天, 祁惠, 杨锁宁, 等. 八仙花萼片有色原生质体游离及应用[J]. 中国农业科技导报, 2022, 24(9): 50-57.
|
LIEPMAN A H, WIGHTMAN R, GESHI N M, et al. Arabidopsis-a Powerful Model System for Plant Cell Wall Research[J]. The Plant Journal, 2010, 61(6): 1107-1121.
|
LIONETTI V, CERVONE F, DE LORENZO G. A Lower Content of De-Methylesterified Homogalacturonan Improves Enzymatic Cell Separation and Isolation of Mesophyll Protoplasts in Arabidopsis[J]. Phytochemistry, 2015, 112: 188-194.
|
JIA X Y, ZHANG X H, QU J M, et al. Optimization Conditions of Wheat Mesophyll Protoplast Isolation[J]. Agricultural Sciences, 2016, 7(12): 850-858.
|
WANG H L, WANG W, ZHAN J C, et al. An Efficient PEG-Mediated Transient Gene Expression System in Grape Protoplasts and Its Application in Subcellular Localization Studies of Flavonoids Biosynthesis Enzymes[J]. Scientia Horticulturae, 2015, 191: 82-89.
|
GAO Y L, TANG T Y, CAO W H, et al. Protoplast Transient Transformation Facilitates Subcellular Localization and Functional Analysis of Walnut Proteins[J]. Plant Physiology, 2025, 197(2): kiae627.
|
GROSSER J W, GMITTER F G J R. Protoplast Fusion and Citrus Improvement[J]. Plant Breed Reviews, 1990, 8: 339-374.
|
DU J G, ZHANG H T, LI W L, et al. Optimization of Protoplast Preparation System from Leaves and Establishment of a Transient Transformation System in Apium graveolens[J]. Agronomy, 2023, 13(8): 2154.
|
TAN B Y, XU M, CHEN Y, et al. Transient Expression for Functional Gene Analysis Using Populus Protoplasts[J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2013, 114(1): 11-18.
|
LI S F, ZHAO R, YE T W, et al. Isolation, Purification and PEG-Mediated Transient Expression of Mesophyll Protoplasts in Camellia oleifera[J]. Plant Methods, 2022, 18(1): 141.
|
MAZAREI M, AL-AHMAD H, RUDIS M R, et al. Protoplast Isolation and Transient Gene Expression in Switchgrass, Panicum virgatum L[J]. Biotechnology Journal, 2008, 3(3): 354-359.
|
张宁, 李威, 顾钊宇, 等. '富士'苹果花粉原生质体分离初探[J]. 园艺学报, 2015, 42(6): 1167-1174.
|
高成昱, 王艺衡, 靳江周, 等. 梨叶片原生质体制备方法的建立及其基因瞬时转化试验[J]. 园艺学报, 2023, 50(5): 1141-1150.
|