WU Juan, LI Jiayan, LI Wei, et al. Analysis of Virus Content and Symptoms of Cucumber Mosaic Virus-based Gene Silencing Vector[J]. PLANT HEALTH AND MEDICINE, 2023, (6): 21-27. doi: 10.13718/j.cnki.zwyx.2023.06.003
| Citation: |
WU Juan, LI Jiayan, LI Wei, et al. Analysis of Virus Content and Symptoms of Cucumber Mosaic Virus-based Gene Silencing Vector[J]. PLANT HEALTH AND MEDICINE, 2023, (6): 21-27. doi: 10.13718/j.cnki.zwyx.2023.06.003
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Analysis of Virus Content and Symptoms of Cucumber Mosaic Virus-based Gene Silencing Vector
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Institute of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi Hunan 417000, China
More Information
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Received Date:
23/09/2023
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MSC:
S432
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Abstract
Virus-induced gene silencing (VIGS) has been widely used for plant gene functional analysis. It is easy to operate, to observe the silencing effect in short time and low cost. Cucumber Mosaic Virus (CMV) has been successfully deployed as a VIGS vector in plants such as chili, tobacco and soybean, playing an important role in plant gene functional analysis. In this study, GFP gene fragments of different lengths were inserted into the 2b gene deletion vector CMV-F209△2b and CMV-F2092b61aa with truncated 2b to analyze the virus expression level and induced virus symptoms of these CMV VIGS vectors in Nicotiana benthamiana. The results indicated that the accumulation of CP RNA was significantly reduced after inserting foreign gene fragments. The CP RNA contents of recombinant viruses CMVF2092b61aa-gfp100, CMVF2092b61aa-gfp200, and CMVF2092b61aa-gfp350 carrying different length of GFP sequences were about 10 times higher than that of CMVF209△2b-gfp350 carrying the optimal length of GFP sequence, and they also did not cause obvious virus symptoms. Therefore, CMVF2092b61aa is more suitable as an efficient VIGS vector than CMV-F209△2b, and its optimal fragment length of insertion has been preliminarily determined to be 200~350 nt.
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