施用竹炭对卷心菜叶绿素含量和荧光参数的影响
- 西南大学/三峡库区生态环境教育部重点实验室,重庆,400715 西南大学/三峡库区生态环境教育部重点实验室,重庆 400715; 浙江农林大学林业与生物技术学院,浙江临安311300 浙江农林大学林业与生物技术学院,浙江临安,311300
摘要: 为探究人工施用竹炭对卷心菜叶绿素含量和荧光参数的影响,进而明确卷心菜的最适竹炭有机肥施用量,在田间试验条件下设置了无碳(CK)、中碳(MC)和高碳(SC)3个处理,对卷心菜莲座期和结球期的叶绿素含量及荧光参数进行测量研究.结果表明:(1)结球期的卷心菜叶绿素含量极显著高于莲座期(p<0.01),且随着竹炭量的增加,两个时期的叶绿素含量变化趋势一致,从高到低依次为:MC ,CK ,SC .(2)结球期的卷心菜初始最小荧光Fo 、初始最大荧光 Fm 、PSⅡ的最大光化学效率 Fv/Fm ,PSⅡ的潜在活性 Fv/Fo 、非光化学猝灭系数q N均低于莲座期,而 PSⅡ实际光化学效率ΦPSⅡ、光化学猝灭系数 q P高于莲座期,且随着竹炭量的增加,两个时期的这些参数均表现出一致的变化趋势,Fo 和 qN 均呈“V”型变化,而 Fm ,Fv/Fm ,Fv/Fo ,ΦPSⅡ和 qP 均呈现倒“V”趋势,但对ΦPSⅡ和 qP没有显著影响(p>0.05).说明结球期的卷心菜光合作用高于莲座期;当竹炭量由CK增加到MC ,有利于提高叶片叶绿素含量,增强光能利用率,减少植株热耗散,提高植株光合作用;但随着竹炭量进一步增加到SC ,卷心菜叶片叶绿素含量降低,光能利用率减弱,热耗散增加,光合作用受到抑制,即中炭(M C )处理条件下,此卷心菜长势最优,表明中炭是该卷心菜生长的最适竹炭有机肥施用量.
Influence of Bamboo Charcoal Application on Chlorophyll Content and Fluorescence Parameters of Cabbage
- 西南大学/三峡库区生态环境教育部重点实验室,重庆,400715 西南大学/三峡库区生态环境教育部重点实验室,重庆 400715; 浙江农林大学林业与生物技术学院,浙江临安311300 浙江农林大学林业与生物技术学院,浙江临安,311300
Keywords:
- 竹炭 /
- 卷心菜 /
- 叶绿素含量 /
- 荧光参数 /
- 有机农业
Abstract: To reveal the effects of bamboo charcoal application on cabbage chlorophyll content and fluores‐cence parameters and to determine the optimum rate of charcoal organic fertilizer for cabbage ,three treat‐ments ,i.e. control (CK) ,moderate charcoal (MC) and severe charcoal (SC) ,were made in a field test , and chlorophyll content (SPAD value) and fluorescence parameters of the leaves at the rosette and heading periods of the plants were measured .Chlorophyll content was highly significantly higher at the heading pe‐riod than at the rosette period (pCK>SC .The initial minimum fluores‐cence Fo ,the initial maximum fluorescence Fm ,the maximum photochemical efficiency of PSⅡ Fv/Fm , the potential activity of PSⅡ Fv/Fo and non‐photochemical quenching coefficient qN at the heading period were lower than those at the heading period ,but PSⅡ actual photochemical efficiency ΦPSⅡ and photo‐chemical quenching qP at the heading period were higher than those at the rosette period .Increased bam‐boo charcoal application resulted in a similar trend in the above seven parameters at both periods ,i.e. Fo and qN exhibited a V‐shaped variation ,and Fm , Fv/Fm , Fv/Fo ,ΦPS Ⅱ and qP an inverted V‐shaped variation ,but there was no significance for ΦPSⅡ or qP (p>0.05) .These results indicated that the pho‐tosynthesis at the heading period was higher than that at the rosette period .Increasing bamboo charcoal application rate from CK to MC contributed to raising chlorophyll content ,enhancing energy efficiency , reducing plant heat dissipation and improving plant photosynthesis .However ,with further increase in charcoal rate to SC ,chlorophyll content and energy use rate decreased ,heat dissipation increased and ,as a result ,photosynthesis was inhibited .In conclusion ,cabbage grew most vigorously in the Treatment MC in this experiment ,indicating that MC is the optimum application rate of bamboo charcoal manure in cabbage production .